Solubilization and resolution of thromboxane synthesizing system from microsomes of bovine blood platelets.
Solubilization and resolution of thromboxane synthesizing system from microsomes of bovine blood platelets.
复制标题
牛血小板微粒体血栓素合成系统的溶解和解析。
DOI：
10.1016/s0021-9258(17)40104-9
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
O. Hayaishi
中科院分区：
文献类型：
--
作者：
T. Yoshimoto;S. Yamamoto;M. Okuma;O. Hayaishi
经过Triton X-100处理，牛血小板微粒体的血栓素合成酶系统被溶解。通过DEAE-纤维素色谱法将溶解的制剂分离成两个酶级分。其中一种在血红素和色氨酸存在下催化花生四烯酸形成前列腺素 H2。另一部分将前列腺素 H2 转化为血栓素 B2 和 12L-羟基-5,8,10-十七碳三烯酸。然而，后一级分与前列腺素H2在较低温度下温育产生了具有血小板聚集活性的不稳定化合物，其可能是血栓素A2并且容易分解为血栓素B2和12L-羟基-5,8,10-十七碳三烯酸。
The thromboxane synthetase system of the microsomes of bovine blood platelets was solubilized by the treatment with Triton X-100. The solubilized preparation was separated into two enzyme fractions by DEAE-cellulose chromatography. One catalyzed the formation of prostaglandin H2 from arachidonic acid in the presence of heme and tryptophan. The other fraction converted prostaglandin H2 to thromboxane B2 and 12L-hydroxy-5,8,10-heptadecatrienoic acid. However, incubation of the latter fraction with prostaglandin H2 at lower temperature produced an unstable compound with platelet-aggregating activity, which was presumably thromboxane A2 and which decomposed readily to thromboxane B2 and 12L-hydroxy-5,8,10-heptadecatrienoic acid.