Identification of the multi-resistance gene cfr in Escherichia coli isolates of animal origin.
Identification of the multi-resistance gene cfr in Escherichia coli isolates of animal origin.
复制标题
DOI：
10.1371/journal.pone.0102378
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liao XP
中科院分区：
文献类型：
--
作者：
Deng H;Sun J;Ma J;Li L;Fang LX;Zhang Q;Liu YH;Liao XP
以往研究表明，多重耐药基因cfr主要存在于葡萄球菌、肠球菌等革兰氏阳性菌中，在大肠埃希菌中零星检出。cfr在大肠杆菌中的流行情况及传播机制尚不清楚。杆菌在本研究中，cfr在E.对2010 - 2012年从广东省食品动物中采集的大肠杆菌分离株进行了调查，并对cfr阳性的大肠杆菌进行了检测。大肠杆菌分离株进行了PFGE，质粒谱，遗传环境分析。在839 E。10株猪源分离株为CFR阳性。PFGE结果显示，所有的cfr阳性菌株均表现为多重耐药表型，且在遗传上存在差异。10株菌株中有8株的cfr基因位于约30 kb的质粒上。经EcoRI酶切和cfr特异性探针序列杂交分析，发现cfr基因片段大小在6~23 kb之间，而在约30 kb的质粒中，cfr基因片段大小一般在18 kb左右。检测到四种不同的cfr遗传环境，其中cfr两侧是两个相同的IS26拷贝，其可以通过同源重组环出插入序列。在8个大小约30 kb的质粒中，有7个质粒的遗传环境相同。这些结果证明了质粒携带的cfr在E.结果表明，在大肠杆菌中，IS 26介导的转座和同源重组可能是cfr基因在大肠杆菌中转移的主要途径。杆菌
Previous study indicated that the multi-resistance gene cfr was mainly found in gram-positive bacteria, such as Staphylococcus and Enterococcus, and was sporadically detected in Escherichia coli. Little is known about the prevalence and transmission mechanism of cfr in E. coli. In this study, the presence of cfr in E. coli isolates collected during 2010–2012 from food-producing animals in Guangdong Province of China was investigated, and the cfr-positive E. coli isolates were characterized by PFGE, plasmid profiling, and genetic environment analysis. Of the 839 E. coli isolates, 10 isolates from pig were cfr positive. All the cfr-positive isolates presented a multi-resistance phenotype and were genetically divergent as determined by PFGE. In 8 out of the 10 strains, the cfr gene was located on plasmids of ∼30 kb. Restriction digestion of the plasmids with EcoRI and sequence hybridization with a cfr-specific probe revealed that the cfr-harboring fragments ranged from 6 to 23 kb and a ∼18 kb cfr-carrying fragment was common for the plasmids that were ∼30 kb. Four different genetic environments of cfr were detected, in which cfr is flanked by two identical copies of IS26, which may loop out the intervening sequence through homologous recombination. Among the 8 plasmids of ∼30 kb, 7 plasmids shared the same genetic environment. These results demonstrate plasmid-carried cfr in E. coli and suggest that transposition and homologous recombination mediated by IS26 might have played a rule in the transfer of the cfr gene in E. coli.
登录
查看更多内容
影响因子：
4.9
作者：
Smith, Lisa K.;Mankin, Alexander S.
通讯作者：
Mankin, Alexander S.
家畜源性大肠杆菌中葡萄球菌多重耐药基因cfr的检测
影响因子：
5.2
作者：
Wang, Yang;He, Tao;Shen, Jianzhong
通讯作者：
Shen, Jianzhong
中国猪中分离出产 RmtB 甲基化酶的大肠杆菌和阴沟肠杆菌。
影响因子：
5.2
作者：
Chen, Lin;Chen, Zhang-Liu;Jiang, Hong-Xia
通讯作者：
Jiang, Hong-Xia
影响因子：
4.9
作者：
Kehrenberg, C;Schwarz, S
通讯作者：
Schwarz, S
影响因子：
5.2
作者：
Liu, Yang;Wang, Yang;Shen, Jianzhong
通讯作者：
Shen, Jianzhong