Effects of electroacupuncture on luteal regression and steroidogenesis in ovarian hyperstimulation syndrome model rat
Effects of electroacupuncture on luteal regression and steroidogenesis in ovarian hyperstimulation syndrome model rat
复制标题
电针对卵巢过度刺激综合征模型大鼠黄体退化及类固醇生成的影响
DOI：
10.1016/j.lfs.2018.01.026
复制
发表时间：
2018-03-15
期刊：
影响因子：
6.1
通讯作者：
Yao, Bing
中科院分区：
文献类型：
--
作者：
Huang, Xuan;Chen, Li;Yao, Bing
目的：电针是一种安全有效的治疗方法，广泛应用于临床疾病的治疗。以往研究发现，电针可降低卵巢过度刺激综合征（OHSS）大鼠模型的血清激素水平，缩小卵巢体积。材料与方法：HE染色计数黄体数和卵泡数。免疫组化和ELISA法检测黄体功能和结构退化。用免疫沉淀法分析NPY与神经肽Y的相互作用结果表明：电针组卵巢重量和CL数明显减轻，卵巢和血清PGE 2和考克斯-2表达水平明显降低，卵巢PGF 2 α水平和PGF 2 α/PGE 2比值明显升高，卵巢组织中PGF 2 α/PGE 2比值明显升高，卵巢组织中PGF 2 α/PGE 2比值明显降低，卵巢组织中PGF 2 α/PGE 2比值明显升高，卵巢组织中PGF 2 α/PGE 2比值明显升高，卵巢组织中PGF 2 α/PGE 2比值明显升高，卵巢组织中PGF 2 α/PGE 2比值明显降低。PCNA表达和分布减少;细胞周期蛋白调节抑制剂p27表达增加，进一步影响黄体形成，促进黄体功能和结构退化。卵巢组织中考克斯-2的表达与NPY的表达具有交互性。电针还能降低血清激素水平，抑制PKA/CREB通路，减少类固醇生成酶的表达。因此，神经肽Y与考克斯-2的相互作用可能会影响前列腺素F 2 α和前列腺素E 2的水平，并影响卵巢颗粒细胞的增殖，从而进一步减少黄体的形成，促进黄体结构和功能的退化，以及EA治疗后卵巢类固醇的生成。意义：EA治疗可能是预防ART中OHSS的一种选择。
Aims: Electroacupuncture (EA) is an effective and safe therapeutic method widely used for treating clinical diseases. Previously, we found that EA could decrease serum hormones and reduce ovarian size in ovarian hyperstimulation syndrome (OHSS) rat model. Nevertheless, the mechanisms that contribute to these improvements remain unclear.Materials and methods: HE staining was used to count the number of corpora lutea (CL) and follicles. Immunohistochemical and ELISA were applied to examine luteal functional and structural regression. Immunoprecipitation was used for analyzing the interaction between NPY (neuropeptide Y) and COX-2; western blotting and qRT-PCR were used to evaluate the expressions of steroidogenic enzymes and PKA/CREB pathway.Key findings: EA treatment significantly reduced the ovarian weight and the number of CL, also decreased ovarian and serum levels of PGE2 and COX-2 expression; increased ovarian PGF2 alpha levels and PGF2 alpha/PGE2 ratio; decreased PCNA expression and distribution; and increased cyclin regulatory inhibitor p27 expression to have further effect on the luteal formation, and promote luteal functional and structural regression. Moreover, expression of COX-2 in ovaries was possessed interactivity increased expression of NPY. Furthermore, EA treatment lowered the serum hormone levels, inhibited PKA/CREB pathway and decreased the expressions of steroidogenic enzymes. Hence, interaction with COX-2, NPY may affect the levels of PGF2 alpha and PGE2 as well as impact the proliferation of granulosa cells in ovaries, thus further reducing the luteal formation, and promoting luteal structural and functional regression, as well as the ovarian steroidogenesis following EA treatment.Significance: EA treatment could be an option for preventing OHSS in ART.