Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage.
Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage.
复制标题
X型胶原蛋白表达的比较研究在正常和rachitic鸡外骨软骨中。
DOI：
10.1083/jcb.109.4.1849
复制
发表时间：
1989-10
影响因子：
7.8
通讯作者：
Grant, M E
中科院分区：
文献类型：
--
作者：
Kwan, A P;Dickson, I R;Freemont, A J;Grant, M E
测定并比较了正常鸡骨膜矿化和非矿化鸡胫骨骨痂中X型胶原的含量。用抗鸡X型胶原的单抗对正常和脊椎病软骨切片进行免疫组织化学的定性研究，结果表明，脊椎病生长板中的X型胶原水平降低。RNA的Northern杂交和生物合成研究已经证实，软骨病中X型胶原的合成也减少。在低钙血症组，X型胶原基因表达水平降低了80%，而在正常钙血症组中，X型胶原基因表达水平仅降低了50%。这些观察结果为X型胶原参与软骨矿化过程提供了进一步的证据，也提示正常钙血症性软骨病X型胶原合成的部分恢复可能与血浆钙浓度升高有关。因此，钙离子浓度可能在控制X型胶原合成中起重要作用。
The levels of type X collagen in mineralizing normal chicken epiphyses and nonmineralizing rachitic chicken tibial epiphyses were measured and compared. Qualitative immunoperoxidase studies with anti-chick type X collagen monoclonal antibodies on sections from normal and rachitic cartilage demonstrated that the type X collagen levels in rachitic growth plates are reduced. Northern hybridization of mRNA and biosynthetic studies have confirmed that type X collagen synthesis in rickets is also decreased. In hypocalcemic rickets, the level of type X collagen mRNA is reduced by 80% whereas the level of type X collagen mRNA is only reduced by 50% in normocalcemic rickets. These observations provide additional evidence that type X collagen is involved in the process of cartilage mineralization and also suggest that the partial recovery of type X collagen synthesis in normocalcemic rickets may be related to the elevated plasma concentration of calcium. Calcium concentration may therefore play an important role in the control of type X collagen synthesis.