Kinetics and mechanisms of p-nitrophenol biodegradation by Pseudomonas aeruginosa HS-D38
Kinetics and mechanisms of p-nitrophenol biodegradation by Pseudomonas aeruginosa HS-D38
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铜绿假单胞菌 HS-D38 生物降解对硝基苯酚的动力学和机制
DOI：
10.1016/s1001-0742(08)62403-1
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发表时间：
2009-01-01
影响因子：
6.9
通讯作者：
Xiong Li
中科院分区：
文献类型：
--
作者：
Zheng Yongliang;Liu Deli;Xiong Li
研究了铜绿假单胞菌HS-D38降解对硝基苯酚（PNP）的动力学和机理。HS-D38菌株能以PNP为唯一碳源、氮源和能源，在无机盐培养基（MSM）中PNP的最大矿化浓度为500 mg/L，24 h内完成矿化。结果表明，PNP的生物降解符合一级动力学模型。在MSM培养基中，速率常数k（PNP）为2.039 × 10（-2）/h，KPNP+N在添加氯化铵的情况下为3.603 × 10（-2）/h，KPNP+C在添加葡萄糖的情况下为9.74 × 10（-3）/h。氯化铵的加入促进了PNP的降解。薄层色谱（TLC）、紫外-可见光谱（UV-Vis）和气相色谱（GC）分析结果表明，PNP经1，2，4-苯三醇（1，2，4-BT）途径降解为对苯二酚（HQ）。
The kinetics and mechanisms of p-nitrophenol (PNP) biodegradation by Pseudomonas aeruginosa HS-D38 were investigated. PNP could be used by HS-D38 strain as the sole carbon, nitrogen and energy sources, and PNP was mineralized at the maximum lconcentration of 500 mg/L within 24 h in an mineral salt medium (MSM). The analytical results indicated that the biodegradation of PNP fit the first order kinetics model. The rate constant k(PNP) is 2.039 x 10(-2)/h in MSM medium, KPNP+N is 3.603 x 10(-2)/h with the addition of ammonium chloride and KPNP+C is 9.74 x 10(-3) /h with additional glucose. The addition of ammonium chloride increased the degradation of PNP. On the contrary, the addition of glucose inhibited and delayed the biodegradation of PNP Chemical analysis results by thin-layer chromatography (TLC), UV-Vis spectroscopy and gas chromatography (GC) techniques suggested that PNP was converted to hydroquinone (HQ) and further degraded via 1,2,4-benzenetriol (1,2,4-BT) pathway.