Hepatocyte growth factor and its receptor c-Met in rat and rabbit vocal folds
Hepatocyte growth factor and its receptor c-Met in rat and rabbit vocal folds
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DOI：
10.1177/000348940211100801
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发表时间：
2002-08-01
影响因子：
1.4
通讯作者：
Kanemaru, SI
中科院分区：
文献类型：
--
作者：
Hirano, S;Thibeault, S;Kanemaru, SI
声带纤维化瘢痕的特征是固有层和上皮纤维化，并且难以治疗。肝细胞生长因子（HGF）具有抗纤维化活性，并且作为治疗纤维化的可能的治疗替代物而受到关注。本研究采用免疫组化和酶联免疫吸附试验检测大鼠声带组织中HGF及其受体c-Met的表达，并检测损伤声带组织中HGF的活性，以探讨HGF是否参与声带瘢痕形成。我们发现HGF和c-Met在脂肪声带的上皮细胞和腺细胞上。在兔声带损伤后，几乎没有观察到肝细胞生长因子损伤后立即，但显着的活动发生在第10至15天的声带粘膜上皮再生的同时。在固有层中的成纤维细胞以及上皮细胞上观察到HGF的活性。提示HGF在声带中由成纤维细胞产生并传递到上皮细胞，提示HGF参与声带的伤口加热，为声带瘢痕的防治提供了一种新的途径。
Vocal fold fibrotic scar is characterized by fibrosis of the lamina propria and epithelium, and is difficult to treat. Hepatocyte growth factor (HGF) has antifibrotic activity and has received attention as a possible therapeutic alternative to treat fibrosis. In this study, in order to clarify whether HGF can be involved in vocal fold scarring, we examined the existence of HGF and its receptor, c-Met, in rat vocal folds, and then the activity of HGF in rabbit injured vocal folds, using immunohistochemistry and enzyme-linked immunosorbent assay. We found HGF and c-Met on epithelial cells and gland cells of the fat vocal folds. On the injured vocal folds of rabbits, little HGF was observed immediately after injury, but prominent activity occurred simultaneously with reepithelialization of the vocal fold mucosa on days 10 to 15. The activity of HGF was observed on fibroblasts in the lamina propria, as well as the epithelium. It is suggested that HGF in the vocal folds is produced by the fibroblasts and delivered to the epithelium, The implication of these findings is that HGF is involved in wound heating of the vocal fold, and may provide an alternative approach in preventing and treating vocal fold scarring.