Long-term cultivation of in vitro Apis mellifera cells by gene transfer of human c-myc proto-oncogene
Long-term cultivation of in vitro Apis mellifera cells by gene transfer of human c-myc proto-oncogene
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DOI：
10.1007/s11626-011-9431-6
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发表时间：
2011-08-01
影响因子：
2.1
通讯作者：
Matsuda, Satoru
中科院分区：
文献类型：
--
作者：
Kitagishi, Yasuko;Okumura, Naoko;Matsuda, Satoru
建立代表蜜蜂特征的细胞系将大大有助于它们的分析。在这里，我们表明，永生化细胞系，命名为MYN 9，已产生从蜜蜂胚胎的基因转移的人c-myc原癌基因。细胞的形态是胚胎干细胞的特征，尽管细胞是稳定的并且不自发分化。聚合酶链反应分析表明，该细胞来源于真实的蜜蜂细胞。将人c-myc基因整合到蜜蜂前体群体中，可建立稳定的细胞系，用于细胞和分子生物学研究。
Establishment of cell lines representative of honeybee character would greatly assist in their analysis. Here, we show that immortalized cell line, designated as MYN9, has been generated from honeybee embryo by the gene transfer of human c-myc proto-oncogene. The morphology of the cell is characteristic of embryonic stem cell, although the cell is stable and does not spontaneously differentiate. Polymerase chain reaction analyses show that the cell is originated from authentic honeybee cell. It is proposed that the integration of human c-myc gene into honeybee precursor populations results in the establishment of stable cell line suitable for cellular and molecular studies.