Cloning of cDNAs encoding G protein-coupled receptor expressed in human endothelial cells exposed to fluid shear stress
Cloning of cDNAs encoding G protein-coupled receptor expressed in human endothelial cells exposed to fluid shear stress
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DOI：
10.1006/bbrc.1997.7734
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发表时间：
1997-11-26
影响因子：
3.1
通讯作者：
Ando, J
中科院分区：
文献类型：
--
作者：
Takada, Y;Kato, C;Ando, J
为了寻找在流体剪切力作用下表达的内皮细胞功能基因，构建了人脐静脉内皮细胞cDNA文库，并利用简并寡核苷酸引物通过聚合酶链反应（PCR）方法克隆了G蛋白偶联受体（GPCR）家族成员的cDNA。另一个克隆是一个名为FEG-1的新基因，它编码一种375个氨基酸的蛋白质，类似于血管紧张素II和趋化因子的受体。RT-PCR结果显示，血流刺激后，内皮细胞FEG-1和edg-1mRNA水平明显升高，提示FEG-1和edg-1可能是生理性血流条件下内皮细胞功能调节的重要受体基因。(C)1997年学术出版社。
A cDNA library of human umbilical vein endothelial cells exposed to fluid shear stress was constructed to search for functional endothelial genes expressed under flow conditions, and cDNAs encoding members of the G protein-coupled receptor (GPCR) family were cloned by a polymerase chain reaction (PCR) method using degenerate oligonucleotide primers, One of the two GPCR clones obtained was edg-1, and the other clone is a novel gene named FEG-1 that encodes a 375-amino acid protein similar to the receptors for both angiotensin II and chemokines. Reverse transcriptase-PCR showed that the FEG-1 and edg-1 mRNA levels in endothelial cells increased markedly in response to fluid flow, This suggests that FEG-1 and edg-1 may be receptor genes that play important roles in the regulation of endothelial function under physiological blood how conditions. (C) 1997 Academic Press.