Developmental potential of bovine androgenetic and parthenogenetic embryos: A comparative study
Developmental potential of bovine androgenetic and parthenogenetic embryos: A comparative study
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DOI：
10.1095/biolreprod.103.021972
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发表时间：
2004-02-01
影响因子：
3.6
通讯作者：
Galli, C
中科院分区：
文献类型：
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作者：
Lagutina, I;Lazzari, G;Galli, C
本研究比较了不同方法获得的牛单倍体和二倍体雄核发育胚胎和孤雌胚胎的发育能力。去核卵母细胞通过压电卵母细胞胞浆内单精子注射(ICSI)或体外受精(IVF)获得雄核发育胚胎，并将其从一个单倍体受精卵移植到另一个单倍体受精卵。用离子霉素联合放线菌素或6-二甲氨基嘌呤(DMAP)激活成熟卵母细胞，获得孤雌胚胎。只有少数雄性发育的单倍体胚胎能够致密(2.7%)并形成囊胚(1.8%)，而更多的单倍体孤雌生殖胚胎发生致密(24-37%)，少数发育到囊胚的比率因激活程序的不同而不同(放线菌胺3%，6-DMAP 14.5%)。相比之下，二倍体雄核、克隆雄核发育胚胎和孤雌生殖胚胎的囊胚率(分别为31%、39%和43%)与体外受精对照胚胎(35%)相似。体外受精囊胚第7天细胞数最高，其次为二倍体雄核胚胎、二倍体孤雌生殖胚胎和单倍体单亲胚胎。在移植二倍体雄核发育胚胎后，妊娠建立并维持到第28天。
In this study, we compared the developmental capacity of bovine haploid and diploid androgenetic and parthenogenetic embryos obtained by different methods. Androgenetic embryos were produced by piezo-intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF) of enucleated oocytes with or without subsequent pronuclear transfer from one haploid zygote to another. Parthenogenetic embryos were obtained by activation of matured oocytes by ionomycin combined with cycloheximide or 6-dimethylaminopurine (DMAP) treatment. Only few cleaved androgenetic haploid embryos were able to compact (2.7%) and to form blastocysts; (1.8%), while significantly more haploid parthenogenotes underwent compaction (24-37%) and a minority developed to blastocysts at different rates, depending on the activation procedure (cycloheximide 3%, 6-DMAP 14.5%). By contrast, development to blastocyst of diploid androgenotes, cloned androgenetic embryos, and parthenogenotes (31%, 39%, and 43%, respectively) was similar to IVF control embryos (35%). Cell number on Day 7 was higher for IVF blastocysts and decreased in consecutive order in diploid androgenotes, diploid parthenogenotes, and haploid uniparental embryos. Following transfer of diploid androgenetic embryos, a pregnancy was established and maintained up to Day 28.