DNA Copy Number Analysis on Tissue Microarrays
DNA Copy Number Analysis on Tissue Microarrays
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DOI：
10.1007/978-1-60761-806-5_13
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发表时间：
2010-01-01
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影响因子：
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通讯作者：
Kallioniemi, Anne
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文献类型：
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作者：
Kallioniemi, Anne
DNA序列拷贝数变化的检测在临床实践和基础研究中都是必不可少的，特别是在癌症研究中。荧光原位杂交（FISH）和组织微阵列（TMA）技术的结合为大量组织样本中的遗传畸变评估提供了高通量手段。TMA上的FISH在技术上要求很高，已经开发了包括各种组织预处理步骤的几种方案来提高这种方法的成功率。尽管技术上存在困难，但对TMA的FISH分析已成功地用于不仅揭示各种恶性肿瘤中的遗传改变，而且还快速确定这些改变的临床意义。
Detection of DNA sequence copy number changes is essential in both clinical practice and basic research, especially in cancer research. The combination of fluorescence in situ hybridization (FISH) and tissue microarray (TMA) technology provides high-throughput means for the evaluation of genetic aberrations in a large number of tissue samples. FISH on TMA is technically demanding and several protocols that include a variety of tissue pretreatment steps have been developed to improve the success of this methodology. Despite of the technical difficulties, FISH analysis on TMA has been successfully used not only to uncover genetic alterations in various malignancies but to also rapidly establish the clinical significance of such changes.