Molecular characterization, expression and association analysis of the porcine CMYA4 gene with carcass traits.
Molecular characterization, expression and association analysis of the porcine CMYA4 gene with carcass traits.
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DOI：
10.1111/j.1439-0388.2008.00719.x
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发表时间：
2008-08
期刊：
影响因子：
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通讯作者：
X. L. Xu;K. Li;Zhongzhen Peng;Shumiao Zhao;M. Yu;Bin Fan;M. Zhu;S. P. Xu;Y. Du;Bang Liu
中科院分区：
文献类型：
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作者：
X. L. Xu;K. Li;Zhongzhen Peng;Shumiao Zhao;M. Yu;Bin Fan;M. Zhu;S. P. Xu;Y. Du;Bang Liu
CMYA4（心肌病相关4）基因在粗纤维组装中起重要作用。本研究采用cDNA末端快速扩增和逆转录聚合酶链式反应（RT-PCR）技术，获得了猪CMYA4基因的完整编码序列和部分5′非翻译区mRNA序列，并将该序列存入GenBank核苷酸数据库（DQ_286571）。人类（NM_173167）和小鼠（NM_178680）同源物与猪CMYA4基因的同源性分别为91%和87%。该序列包含一个编码930个氨基酸残基的开放阅读框，预测的CMYA4蛋白的氨基端包含3个串联重复序列，属于四肽重复家族。半定量RT-PCR结果显示，猪CMYA4基因仅在横纹肌组织中表达。MspI pcr检测到CMYA4内含子15的A558G单核苷酸多态性-限制性片段长度多态性显示，来自6个品种的225头无亲缘关系猪的等位基因频率存在差异。基因型与生长性状和胴体性状的相关性表明，CMYA4基因的不同基因型与第6 ~ 7肋间背膘厚度（p < 0.05）和肩部背膘厚度（p < 0.05）显著相关。
CMYA4 (cardiomyopathy-associated 4) gene plays an important role in thick filament assembly. In this study, we obtained the mRNA sequence including the full coding sequence and the partial 5' untranslated region of the porcine CMYA4 gene by using the rapid amplification of cDNA ends and reverse transcriptase polymerase chain reaction (RT-PCR) and the sequence was deposited in the GenBank nucleotide database (DQ_286571). The human (NM_173167) and mouse (NM_178680) homologues have a 91% and 87% identity with the porcine CMYA4 gene, respectively. The sequence contains an open reading frame encoding 930 amino acid residues, and the amino terminus of the predicted CMYA4 protein contains three tandem repeats belonging to the tetratricopeptide repeat family. Semi-quantitative RT-PCR results showed that the porcine CMYA4 gene is expressed exclusively in striated muscle tissue. An A558G single nucleotide polymorphism in the CMYA4 intron 15 detected as an MspI PCR-restriction fragment length polymorphism showed allele frequency differences among 225 unrelated pigs from six breeds. Association of the genotypes with growth and carcass traits showed that different genotypes of the CMYA4 gene were significantly associated with the backfat thickness of the area between sixth and seventh ribs (p < 0.05) and backfat thickness at the shoulder (p < 0.05).