Adaptive Diversification in the Cellular Circadian Behavior of <i>Arabidopsis</i> Leaf- and Root-Derived Cells
Adaptive Diversification in the Cellular Circadian Behavior of <i>Arabidopsis</i> Leaf- and Root-Derived Cells
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拟南芥叶和根细胞昼夜节律行为的适应性多样化
DOI：
10.1093/pcp/pcac008
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发表时间：
2022
影响因子：
4.9
通讯作者：
Oyama Tokitaka
中科院分区：
文献类型：
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作者：
Nakamura Shunji;Oyama Tokitaka
植物昼夜节律系统基于自我维持的细胞振荡，用于适应日常和季节性环境变化。地上和地下植物器官的细胞生物钟受到不同的局部环境的影响。单个细胞时钟受到植物中其他细胞/组织的影响，并且单个细胞的内在昼夜节律特性仍有待阐明。在这项研究中，我们监测了来自 aCCA1::LUC 拟南芥转基因植物叶和根的单个原生质体衍生细胞的生物发光昼夜节律。我们分析了叶和根来源的细胞的昼夜节律特性，并证明与其他细胞没有物理接触的细胞拥有真正的生物钟，具有约 24 小时的周期性、可夹带性和周期温度补偿。节律的稳定性取决于细胞密度。高细胞密度导致叶源细胞的昼夜节律改善，而无论细胞节律之间的相位关系如何，都能观察到这种效应。对单个细胞生物发光节律的定量和统计分析揭示了叶源细胞和根源细胞之间光/暗夹带的幅度和精度的差异。叶子和根部的昼夜节律系统是多样化的，以适应细胞水平上的局部环境。
The plant circadian system is based on self-sustained cellular oscillations and is utilized to adapt to daily and seasonal environmental changes. The cellular circadian clocks in the above- and belowground plant organs are subjected to diverse local environments. Individual cellular clocks are affected by other cells/tissues in plants, and the intrinsic circadian properties of individual cells remain to be elucidated. In this study, we monitored bioluminescence circadian rhythms of individual protoplast-derived cells from leaves and roots of aCCA1::LUC Arabidopsistransgenic plant. We analyzed the circadian properties of the leaf- and root-derived cells and demonstrated that the cells with no physical contact with other cells harbor a genuine circadian clock with ∼24-h periodicity, entrainability and temperature compensation of the period. The stability of rhythm was dependent on the cell density. High cell density resulted in an improved circadian rhythm of leaf-derived cells while this effect was observed irrespective of the phase relation between cellular rhythms. Quantitative and statistical analyses for individual cellular bioluminescence rhythms revealed a difference in amplitude and precision of light/dark entrainment between the leaf- and root-derived cells. Circadian systems in the leaves and roots are diversified to adapt to their local environments at the cellular level.