Immunolocalization of Odorant-Binding Proteins on Antennal Chemosensilla of the Peach Aphid Myzus persicae (Sulzer)
Immunolocalization of Odorant-Binding Proteins on Antennal Chemosensilla of the Peach Aphid Myzus persicae (Sulzer)
复制标题
桃蚜 Myzus persicae (Sulzer) 触角趋化感受器上气味结合蛋白的免疫定位
DOI：
10.1093/chemse/bjs093
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发表时间：
2013-02-01
期刊：
影响因子：
3.5
通讯作者：
Ban, Li-Ping
中科院分区：
文献类型：
--
作者：
Sun, Yin-Peng;Zhao, Li-Jing;Ban, Li-Ping
利用扫描电镜和透射电镜对桃蚜触角感器进行了定位。在触角的第6节上发现了板形感器和腔锥感器，而在触角的整个长度上发现了两种类型的腹形感器。有翅蚜虫触角第5节上有一个板状感器，第3节上也有板状感器。气味结合蛋白（OBP）的免疫细胞化学定位进行触角化学感受器的切片。OBP7的抗血清强烈标记所有的板状感器从初级和次级紫茎泽兰，金颗粒集中在淋巴周围的树突。OBP6和OBP3也可在板状感器中检测到，但不如OBP7强烈。与针对OBP8的抗体几乎没有观察到显著反应或没有观察到反应。
The antennal sensilla of Myzus persicae were mapped using scanning and transmission electron microscopy. Placoid sensilla and coeloconic sensilla were found on the 6th segments, whereas 2 types of trichoid sensilla were present all through the length of the antenna. A single sensillum placoideum was located on the 5th segment, whereas alate aphids also presented placoid sensilla on the 3rd antennal segment. Immunocytochemical localization of odorant-binding proteins (OBPs) was performed on ultrathin sections of antennal chemosensilla. The antiserum against OBP7 intensively labeled all placoid sensilla from both primary and secondary rhinaria, with gold granules concentrated in the lymph surrounding the dendrite. OBP6 and OBP3 could also be detected in placoid sensilla, but less strongly than OBP7. Barely significant reaction or no reaction was observed with antibodies against OBP8.