A proteomic study on human osteoblastic cells proliferation and differentiation
A proteomic study on human osteoblastic cells proliferation and differentiation
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DOI：
10.1002/pmic.200500858
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发表时间：
2006-06-01
期刊：
影响因子：
3.4
通讯作者：
Santucci, Annalisa
中科院分区：
文献类型：
--
作者：
Spreafico, Adriano;Frediani, Bruno;Santucci, Annalisa
与前成骨细胞（分化标记物）相比，在人类成熟成骨细胞中确定了 17 种蛋白质表达谱的变化。使用差异化方法来突出人骨肉瘤细胞和成熟成骨细胞之间的蛋白质组变化，显示 8 种蛋白质（增殖和肿瘤指标）的相对过度表达，以及在前成骨细胞（成骨细胞分化的特定标记）中也发现下调的蛋白质表达不足。我们的研究结果证实了细胞系和原代人类细胞培养物之间的差异，并建议谨慎使用骨肉瘤来研究人类抗骨质疏松药物。
Changes in expression profiles for 17 proteins were ascertained in human mature osteoblasts compared to pre-osteoblasts (differentiation markers). A differential approach was used to highlight proteomic changes between human osteosarcoma cells and mature osteoblasts, showing a relative over-expression of 8 proteins (proliferation and tumor indicators), as well as underexpression of proteins also found down-regulated in pre-osteoblasts (specific markers of osteoblast differentiation). Our findings confirmed the differences between cell lines and primary human cell cultures and suggested caution on the use of osteosarcoma to study anti-osteoporotic drugs in humans.