Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination
Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination
复制标题
使用方位角旋转照明的具有高空间分辨率的无标记表面敏感光子显微镜
DOI：
10.1126/sciadv.aav5335
复制
发表时间：
2019-03-01
期刊：
影响因子：
13.6
通讯作者：
Zhang, Douguo
中科院分区：
文献类型：
--
作者：
Kuai, Yan;Chen, Junxue;Zhang, Douguo
将SPS和BSWs扩展到显微镜会导致成像伪影，这些伪影可以通过使用方位旋转照明来消除。具有单向照明的表面等离子体共振显微镜(SPRM)因其宽视场、无标记和高表面灵敏度成像能力而成为生物医学成像的强大平台。然而，有两个缺点阻碍了SPRM的更广泛使用。首先是它的空间分辨率很差，可能高达几微米。第二，SPRM要求使用金属薄膜作为样品衬底；这引入了工作波长限制。此外，细胞培养在金属薄膜上的生长并不像在介质衬底上生长那样普遍。在这里，我们展示了方位旋转照明的使用允许SPRM空间分辨率提高高达一个数量级。金属薄膜也可以被电介质多层膜取代，从而发展出一种不同的无标记表面敏感光子显微镜，它在工作波长、偏振和成像部分方面有更多的选择，将为生物应用带来机会。
Extending SPs and BSWs for microscopy leads to imaging artifacts, which are removed by using azimuthal rotation illumination. Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial resolution that can be as large as several micrometers. The second is that SPRM requires use of metal films as sample substrates; this introduces working wavelength limitations. In addition, cell culture growth on metal films is not as universally available as growth on dielectric substrates. Here we show that use of azimuthal rotation illumination allows SPRM spatial resolution to be enhanced by up to an order of magnitude. The metal film can also be replaced by a dielectric multilayer and then a different label-free surface-sensitive photonic microscopy is developed, which has more choices in terms of the working wavelength, polarization, and imaging section, and will bring opportunities for applications in biology.