Regional variations in intestinal brush border membrane fluidity and function during diabetes and the role of oxidative stress and non-enzymatic glycation
Regional variations in intestinal brush border membrane fluidity and function during diabetes and the role of oxidative stress and non-enzymatic glycation
复制标题
DOI：
10.1023/a:1025599126840
复制
发表时间：
2003-10-01
影响因子：
4.3
通讯作者：
Sivakami, S
中科院分区：
文献类型：
--
作者：
Bhor, VM;Sivakami, S
脂质的物理状态（流动性）调节多种膜结合酶和转运蛋白的活性。刷状缘膜（BBM）流动性的改变是糖尿病期间小肠表现出的几种变化之一。在本研究中，研究了糖尿病引起的肠道 BBM 流动性、氧化损伤、非酶糖化以及碱性磷酸酶和 γ-谷氨酰转肽酶的活性和动力学参数的局部变化。在糖尿病治疗 6 周结束时，观察到沿肠道长度的氧化损伤和非酶糖化程度显着增加，同时膜流动性降低。在十二指肠和空肠中观察到 BBM 流动性降低与非酶糖化增加之间存在显着相关性。此外，还观察到两种酶的活性和动力学参数存在区域差异。
The physical state (fluidity) of lipids modulates the activities of several membrane bound enzymes and transport proteins. Alteration of brush border membrane (BBM) fluidity is one of the several changes exhibited by the small intestine during diabetes. In the present study, an investigation of the diabetes induced regional changes in fluidity, oxidative damage, non-enzymatic glycation as well as the activities and the kinetic parameters of the enzymes alkaline phosphatase and gamma-glutamyl transpeptidase was carried out on the intestinal BBM. At the end of 6 weeks of diabetes, significant increases in the extent of both oxidative damage and non-enzymatic glycation were observed along the length of the intestine along with a simultaneous decrease in membrane fluidity. A significant correlation between the decrease in BBM fluidity and increase in non-enzymatic glycation was observed in the duodenum and jejunum. Additionally regional variations in the activities and kinetic parameters of both the enzymes were observed.