Expression of estrogen receptor-beta isoforms in Barrett's metaplasia, dysplasia and esophageal adenocarcinoma.
Expression of estrogen receptor-beta isoforms in Barrett's metaplasia, dysplasia and esophageal adenocarcinoma.
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发表时间：
2004-09
影响因子：
2
通讯作者：
Liang Liu;M. Chirala;M. Younes
中科院分区：
文献类型：
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作者：
Liang Liu;M. Chirala;M. Younes
我们以前已经表明，大多数食管腺癌（EA），及其前体巴雷特化生（BM），表达雌激素受体β（ER-B）。已经描述了ER-B的几种亚型，并推测其具有不同的功能，但其在BM和EA中的分布尚不清楚。这项工作的目的是确定哪些ER-B亚型在EA和BM中表达。对33例食管癌切除标本（其中27例为侵袭性食管癌）的福尔马林固定和石蜡包埋的食管组织切片进行ER-B亚型ER-B1，ER-B2，ER-B3和ER-B5利用免疫过氧化物酶法在27例EA中的23例（85%）中检测到ER-B1，而在14例Barrett化生中的3例（21%）中检测到异型增生阴性（BMND）（p = 0.001）。0. 0001）; ER-B2在27例EA中有22例（81%）表达，而在14例BMND中有3例（21 27例EA患者中27例（100%）ER-B3阳性，而14例BMND患者中仅1例（7%）ER-B3阳性（p<0.001）;在27/27（100%）EA中检测到ER-B5，而在14/14（62%）BMND中检测到ER-B5（p=0.0027）。高度和低度异型增生显示出与EA相似的ER-β亚型表达谱。与局限于食管壁的肿瘤相比，通过食管壁浸润的癌症具有更高百分比的ER-B1胞质表达的细胞（T3 vs. T1和T2，p=0.051）。我们的结论是，ER-B1，ER-B2，ER-B3和ER-B5在EA过表达相比，其前驱病变BMND，这表明在EA的类固醇激素的重要生物学作用。
We have previously shown that the majority of esophageal adenocarcinomas (EA), and its precursor Barrett's metaplasia (BM), express estrogen receptor beta (ER-B). Several isoforms of ER-B have been described and are presumed to have different functions, but their distribution in BM and EA is not known. The aim of this work was to determine which ER-B isoforms are expressed in EA and BM. Sections of formalin-fixed and paraffin-embedded esophageal tissue from 33 esophageactomy specimens, of which 27 had invasive EA, were stained for the ER-B isoforms ER-B1, ER-B2, ER-B3 and ER-B5 utilizing the immunoperoxidase method ER-B1 was detected in 23 out of 27 (85%) EA compared to 3 out of 14 (21%) Barrett's metaplasia negative for dysplasia (BMND) (p =0. 0001); ER-B2 was expressed in 22 out of 27 (81%) EA in contrast to 3 out of 14 (21%) BMND (p=0.0004); ER-B3 was positive in 27 out of 27 (100%) EA in contrast to only 1 out of 14 (7%) BMND (p<0001); ER-B5 was detected in 27 out of 27 (100%) EA compared to 9 out of 14 (62%) of BMND (p=0.0027). High- and low-grade dysplasia showed a similar ER-beta isoform expression profile to that of EA Cancers invasive through the esophageal wall had a higher percent of cells with cytoplasmic expression of ER-B1 than tumors limited to the wall (T3 vs. T1 and T2, p=0.051). We conclude that ER-B1, ER-B2, ER-B3 and ER-B5 are overexpressed in EA compared to its precursor lesion BMND, suggesting a significant biological role for steroid hormones in EA.