Glycans as Biofunctional Ligands for Gold Nanorods: Stability and Targeting in Protein-Rich Media
Glycans as Biofunctional Ligands for Gold Nanorods: Stability and Targeting in Protein-Rich Media
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DOI：
10.1021/jacs.5b01001
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发表时间：
2015-03-18
影响因子：
15
通讯作者：
Liz-Marzan, Luis M.
中科院分区：
文献类型：
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作者：
Garcia, Isabel;Sanchez-Iglesias, Ana;Liz-Marzan, Luis M.
聚（乙二醇）（PEG）已成为生物流体中等离子体纳米颗粒（NP）稳定的金标准，因为它可以防止聚集，同时最大限度地减少与蛋白质的非特异性相互作用。Au NPs在生物环境中的应用需要使用可以靶向所选受体的配体，即使在富含蛋白质的介质存在下。我们在这里证明了低分子量聚糖在生理条件下对球形和棒状等离子体纳米颗粒的稳定作用，以成熟的PEG配体为基准。聚糖包被的纳米颗粒对蛋白质从含血清培养基中的吸附具有抗性，并避免被巨噬细胞样细胞吞噬，但在富含蛋白质的生物培养基中保留对碳水化合物结合蛋白的选择性。这些结果为设计用于特定生物应用的有效治疗/诊断聚糖修饰的等离子体纳米工具开辟了道路。
Poly(ethylene glycol) (PEG) has become the gold standard for stabilization of plasmonic nanoparticles (NPs) in biofluids, because it prevents aggregation while minimizing unspecific interactions with proteins. Application of Au NPs in biological environments requires the use of ligands that can target selected receptors, even in the presence of protein-rich media. We demonstrate here the stabilizing effect of low-molecular-weight glycans on both spherical and rod-like plasmonic NPs under physiological conditions, as bench-marked against the well-established PEG ligands. Glycan-coated NPs are resistant to adsorption of proteins from serum-containing media and avoid phagocytosis by macrophage-like cells, but retain selectivity toward carbohydrate-binding proteins in protein-rich biological media. These results open the way toward the design of efficient therapeutic/diagnostic glycan-decorated plasmonic nanotools for specific biological applications.