Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury
Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury
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DOI：
10.1016/j.apjtm.2016.05.007
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发表时间：
2016-07-01
影响因子：
3.1
通讯作者：
Liu, Jin
中科院分区：
文献类型：
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作者：
Fang, Hua;Zhang, Jing-Chao;Liu, Jin
目的：观察腹主动脉灌注天麻素对脊髓缺血再灌注损伤的保护作用。方法：36只新西兰白兔随机分为假手术组(S组)、对照组(C组)和天麻素组(G组)，每组12只。采用腹主动脉肾下阻断法建立大鼠SCIRI模型。分别记录缺血前、天麻素再灌流30min、60min、6h、12h、24h的运动诱发电位变化，并评定缺血前、再灌流6h、12h、24h的神经功能评分。测定缺血前、缺血45min、天麻素再灌流30min、60min、6h、12、11、24 h血清神经元特异性烯醇化酶(NSE)、白介素1β(IL-1β)、白介素8(IL-8)浓度的变化。检测脊髓神经细胞线粒体超氧化物歧化酶(SOD)、活性氧(ROS)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、总抗氧化能力(T-AOC)和线粒体肿胀度(MSD)，并观察脊髓组织病理变化。结果：C组缺血后NSE、IL-1β、IL-8、ROS、MDA、MSD均显著升高(P
Objective: To observe the effects of perfusion of the gastrodin in abdominal aorta for alleviating the spinal cord ischemia reperfusion injury (SCIRI). Methods: A total of 36 New Zealand white rabbits were divided randomly into sham-operated group (group S), control group (group C) and gastrodin group (group G), 12 rabbits for each group. Aorta abdominalis infrarenalis blocking method was applied to establish the SCIRI model. The changes of motor evoked potentials (MEPs) before the ischemia and on 30 min, 60 min, 6 h, 12 h and 24 h of reperfusion of the gastrodin were respectively recorded, and the neurologic function score before the ischemia, on the 6 h, 12 h and 24 h of the reperfusion of the gastrodin were assessed. And the changes of the concentration of serum neuron specific enolase (NSE), interleukin (IL)-1 beta and IL-8 were measured before the ischemia, after 45 min of ischemia, and on 30 min, 60 min, 6 h, 12 11 and 24 h of reperfusion of gastrodin. Then the levels of spinal cord nerve cells mitochondrial superoxide dismutase (SOD), reactive oxygen species (ROS), glutathione peroxidase (GSH-PX), malondialdehyde (MDA), total antioxidant capacity (T-AOC) and mitochondrial swelling degree (MSD) were tested and the histopathologic changes in spinal cord tissues were observed. Results: The levels of the NSE, IL-1 beta, IL-8, ROS, MDA and MSD of group C were all significantly elevated after the ischemia (P