CYTOPHOTOMETRIC STUDIES ON HAPLOID AND DIPLOID CELLS WITH DIFFERENT DEGREES OF CHROMATIN COILING

CYTOPHOTOMETRIC STUDIES ON HAPLOID AND DIPLOID CELLS WITH DIFFERENT DEGREES OF CHROMATIN COILING
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不同染色质卷曲程度的单倍体和二倍体细胞的细胞光度研究

DOI:
10.1111/j.1749-6632.1969.tb12662.x
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发表时间:
1969
期刊:
影响因子:
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通讯作者:
A. Garcia
A. Garcia
中科院分区:
--
文献类型:
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作者:
A. Garcia

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我们对哺乳动物血液定量细胞化学的兴趣开始于几年前,当时我们在哥伦比亚大学(Columbia University),波利斯特和奥恩斯坦。我们面临着一个当时只受到少数工人注意的问题。我们的目的是确定“第一阶段”(即仪器和测量方法)的误差在多大程度上影响了feulgen染色细胞群体的“真实”扩散。我们使用Pollister-Moses型细胞温度计,用Ornstein和Patau的双波长法测量了人和啮齿动物血液和骨髓的feulgen染色涂片
Our interest in quantitative cytochemistry of mammalian blood started years ago, when we were working at Columbia University under the direction of Drs. AW Pollister and L. Ornstein. We were confronted with a problem which at that time had received attention only from a few~ orkers.~,~**~~ Our aim was to ascertain to what extent errors at the ‘‘first stage”(ie, instrument and method of measurement) influenced the “true” spread of a population of Feulgen-stained cells. We used a Pollister-Moses type of cyto~ hotometer~~ and measured Feulgenstained smears of blood and bone marrow of humans and rodents by means of the two-wavelength method of Ornstein and Patau. 12J3