Improvement of Wuyiencin Biosynthesis in Streptomyces wuyiensis CK-15 by Identification of a Key Regulator, WysR
Improvement of Wuyiencin Biosynthesis in Streptomyces wuyiensis CK-15 by Identification of a Key Regulator, WysR
复制标题
通过鉴定关键调节因子 WysR 改进武夷链霉菌 CK-15 中武夷菌素的生物合成
DOI：
10.4014/jmb.1405.05017
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发表时间：
2014-12-01
影响因子：
2.8
通讯作者：
Zhang, Kecheng
中科院分区：
文献类型：
--
作者：
Liu, Yanyan;Ryu, Hojin;Zhang, Kecheng
武夷菌素是由不吸水链霉菌（Streptomyces ahygroscopicus var. Wuyiensis CK-15，在农业上广泛用作抗真菌剂。对武夷菌素生物合成基因簇的分析揭示了wysR，它是转录调节基因LAL家族的成员。WysR由N-末端PAS结构域和LuxR家族C-末端螺旋-转角-螺旋基序组成。然而，wysR在武夷菌素生物合成中的作用在很大程度上是未知的。在这项研究中，我们发现，wysR的失活导致武夷菌素生产的完全丧失，这可以通过与wysR的单拷贝互补来恢复。此外，我们成功地增加武夷菌素生产到显着更高的水平，通过过度表达的wysR在S。武夷山CK-15。实时荧光定量RT-PCR分析表明，WysR通过调节其他可能的调控基因来调控武夷菌素的生物合成。因此，WysR基因是武夷菌素生物合成的激活因子，过量表达WysR基因是提高武夷菌素产量的有效策略。
Wuyiencin is produced by Streptomyces ahygroscopicus var. wuyiensis CK-15 and is widely used as an antifungal agent in agriculture. Analysis of wuyiencin biosynthetic gene clusters reveals wysR, a member of the LAL-family of transcriptional regulatory genes. WysR consists of an N-terminal PAS domain and a LuxR family C-terminal helix-turn-helix motif. However, the roles of wysR in wuyiencin biosynthesis are largely unknown. In this study, we showed that inactivation of wysR resulted in the complete loss of wuyiencin production, which could be restored by complementation with a single copy of wysR. Furthermore, we successfully increased wuyiencin production to a significantly higher level by overexpression of wysR in S. wuyiensis CK-15. Quantitative real-time RT-PCR analysis showed that WysR regulates wuyiencin biosynthesis by modulating other putative regulatory genes. Thus, WysR was identified as an activator of wuyiencin biosynthesis, and overexpression of wysR gene proved to be an effective strategy for improving wuyiencin production.