Reduction of the severity of canine osteoarthritis by prophylactic treatment with oral doxycycline.
Reduction of the severity of canine osteoarthritis by prophylactic treatment with oral doxycycline.
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通过口服多西环素预防性治疗减轻犬骨关节炎的严重程度。
DOI：
10.1002/art.1780351007
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发表时间：
1992
影响因子：
--
通讯作者：
Brandt,DA
中科院分区：
文献类型：
--
作者：
YuJr,LP;SmithJr,GN;Brandt,KD;Myers,SL;O'Connor,BL;Brandt,DA
目的：体外研究表明，骨关节炎 (OA) 软骨中的中性金属蛋白酶水平升高，并且多西环素 (doxy) 抑制 OA 软骨提取物中的胶原溶解和明胶溶解活性。本研究的目的是测试口服多西对 OA 软骨退变严重程度的影响。方法：在 12 只成年杂种犬背根神经节切除术后 2 周，通过横断前十字韧带 (ACL) 诱导 OA。六只狗从 ACL 横断后的第二天开始口服 doxy，直到 8 周后被处死；另外 6 只作为未经治疗的 OA 对照。结果。每只未经治疗的狗的不稳定膝关节均表现出股骨内侧髁广泛的全层软骨溃疡。与此形成鲜明对比的是，在两只接受 doxy 治疗的狗中，不稳定膝关节股骨髁远端的软骨基本正常，而在其他狗中仅表现出变薄和/或表面不规则。然而，内侧滑车嵴上的退行性软骨损伤、内侧胫骨平台的浅表颤动以及骨赘增生并未受到 doxy 治疗的影响。未经治疗的狗膝骨关节炎的软骨提取物中的胶原蛋白分解活性和明胶分解活性分别比给予 doxy 的狗的提取物高 5 倍和 4 倍。结论：预防性施用 doxy 显着降低了股骨内侧髁承重区域 OA 的严重程度。 OA 发生变化后给予 doxy 是否也有效仍有待确定。
Objective.In vitro studies have indicated that levels of neutral metalloproteinases in osteoarthritic (OA) cartilage are elevated and that doxycycline (doxy) inhibits collagenolytic and gelatinolytic activity in extracts of OA cartilage. The purpose of the present study was to test the effect of oral doxy administration on the severity of cartilage degeneration in OA.Methods.OA was induced in 12 adult mongrel dogs by transection of the anterior cruciate ligament (ACL) 2 weeks after dorsal root ganglionectomy. Six dogs received doxy orally from the day after ACL transection until they were killed 8 weeks later; the other 6 served as untreated OA controls.Results.The unstable knee of each untreated dog exhibited extensive full‐thickness cartilage ulceration of the medial femoral condyle. In sharp contrast, cartilage on the distal aspect of the femoral condyle of the unstable knee was grossly normal in 2 doxy‐treated dogs, and exhibited only thinning and/or surface irregularity in the others. Degenerative cartilage lesions on the medial trochlear ridge, superficial fibrillation of the medial tibial plateau, and osteophytosis were, however, unaffected by doxy treatment. Collagenolytic activity and gelatinolytic activity in cartilage extracts from OA knees of untreated dogs were 5‐fold and 4‐fold greater, respectively, than in extracts from dogs given doxy.Conclusion.Prophylactic administration of doxy markedly reduced the severity of OA in weight‐bearing regions of the medial femoral condyle. It remains to be determined whether administration of doxy after OA changes have developed is also effective.