Thromboxane and stable prostaglandin endoperoxide analogs stimulate water permeability in the toad urinary bladder.
Thromboxane and stable prostaglandin endoperoxide analogs stimulate water permeability in the toad urinary bladder.
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血栓素和稳定的前列腺素内过氧化物类似物刺激蟾蜍膀胱的水渗透性。
DOI：
10.1172/jci109976
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Halushka,PV
中科院分区：
文献类型：
--
作者：
Burch,RM;Halushka,PV
本文研究了血栓素B_2和稳定的前列腺素内过氧化物类似物（15 Z）-羟基-9 α-11 α -（环氧亚甲基）前列腺-5Z，13 E-二烯酸（U44069）和（15 Z）-羟基-11 β，9 α-（环氧亚甲基）前列腺-5Z，13 E-二烯酸（U46619）对蟾蜍膀胱水流量的影响。前列腺素和血栓素A2合成的抑制剂吲哚美辛或甲氨酰胺酸存在时，血栓素B2以剂量依赖的方式刺激水流。U44069（1 μM）刺激水流量从3.6±0.8至12.4±1.2 mg/min/10 cm 2半膀胱表面积，而U46619（1 μM）刺激水流量从2.8±1.0至21.8±2.0 mg/min/10 cm 2。前列腺素内过氧化物/血栓素A2拮抗剂反式-13-氮杂前列腺烷酸，一种加压素刺激的水流抑制剂，以剂量依赖的方式抑制血栓素B2和U46619刺激的水流。失活的顺-13-氮杂前列腺烷酸没有抑制加压素刺激的水流量在未经处理的半膀胱和U46619刺激的水流量在吲哚美辛或甲氰氨酸预处理的半膀胱没有影响。U46619（1 μM）增强了吲哚美辛预处理的半膀胱中加压素刺激的水流量，产生了显著的平行位移（P< 0.001）在与次最大浓度加压素的剂量反应关系中（0.1-0.6 mU/ml），而不影响由超最大浓度的加压素刺激的水流（10 mU/ml）.反式-13-氮杂前列腺烷酸可消除U46619对加压素刺激的水流量的增强作用。这些结果表明血栓烷A2样化合物刺激蟾蜍膀胱中的水流动。
The effects of thromboxane B2and the stable prostaglandin endoperoxide analogs (15Z)-hydroxy - 9α - 11α - (epoxymethano)prosta - 5Z,13E - dienoic acid (U44069) and (15Z)-hydroxy -11α,9α-(epoxymethano) prosta-5Z,13E-dienoic acid (U46619) were tested on water flow across the toad urinary bladder. In the presence of indomethacin or meclofenamic acid, inhibitors of prostaglandin and thromboxane A2synthesis, thromboxane B2stimulated water flow in a dose-dependent manner. U44069 (1 μM) stimulated water flow from 3.6±0.8 to 12.4±1.2 mg/min per 10 cm2hemibladder surface area, while U46619 (1 μM) stimulated water flow from 2.8±1.0 to 21.8±2.0 mg/min per 10 cm2. The prostaglandin endoperoxide/thromboxane A2antagonisttrans- 13-azaprostanoic acid, an inhibitor of vasopressin-stimulated water flow, inhibited thromboxane B2- and U46619-stimulated water flow in a dose-dependent manner. The inactivecis-13-azaprostanoic acid did not inhibit vasopressin-stimulated water flow in untreated hemibladders and had no effect on U46619-stimulated water flow in indomethacin or meclofenamic acid pretreated hemibladders. U46619 (1 μM) enhanced vasopressin-stimulated water flow in indomethacin pretreated hemibladders, producing a significant parallel shift (P< 0.001) in the dose-response relationship to submaximal concentrations of vasopressin (0.1-0.6 mU/ml), while not affecting water flow stimulated by supramaximal concentrations of vasopressin (10 mU/ml).trans-13-Azaprostanoic acid abolished the potentiating effects of U46619 on vasopressin-stimulated water flow. These results show that thromboxane A2-like compounds stimulate water flow in the toad urinary bladder.