Citrate Synthase Is a Novel In Vivo Matrix Metalloproteinase-9 Substrate That Regulates Mitochondrial Function in the Postmyocardial Infarction Left Ventricle
Citrate Synthase Is a Novel In Vivo Matrix Metalloproteinase-9 Substrate That Regulates Mitochondrial Function in the Postmyocardial Infarction Left Ventricle
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DOI：
10.1089/ars.2013.5411
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发表时间：
2014-11-10
影响因子：
6.6
通讯作者：
Lindsey, Merry L.
中科院分区：
文献类型：
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作者：
Bras, Lisandra E. de Castro;Cates, Courtney A.;Lindsey, Merry L.
目的：评估基质金属蛋白酶（MMP）-9 缺失对心肌梗死（MI）后柠檬酸合酶（CS）活性的作用。结果：我们使用基于微分溶解度的方法对左心室 (LV) 样本进行分级。通过质谱分析不溶性蛋白部分，我们确定 CS 是 MI 环境中 MMP-9 的潜在细胞内底物。两种基因型的 MI 后第 1 天，不溶性部分中的 CS 蛋白水平均增加 (p
Aim: To evaluate the role of matrix metalloproteinase (MMP)-9 deletion on citrate synthase (CS) activity postmyocardial infarction (MI). Results: We fractionated left ventricle (LV) samples using a differential solubility-based approach. The insoluble protein fraction was analyzed by mass spectrometry, and we identified CS as a potential intracellular substrate of MMP-9 in the MI setting. CS protein levels increased in the insoluble fraction at day 1 post-MI in both genotypes (p