Identification of α-dystroglycan as a receptor for lymphocytic choriomeningitis virus and lassa fever virus
Identification of α-dystroglycan as a receptor for lymphocytic choriomeningitis virus and lassa fever virus
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DOI：
10.1126/science.282.5396.2079
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发表时间：
1998-12-11
期刊：
影响因子：
56.9
通讯作者：
Oldstone, MBA
中科院分区：
文献类型：
--
作者：
Cao, W;Henry, MD;Oldstone, MBA
从允许感染的细胞中纯化出一种与淋巴细胞脉络膜脑膜炎病毒（LCMV）相互作用的外周膜蛋白。该蛋白质的胰蛋白酶肽被确定为 α-肌营养不良聚糖 (α-DG)。 LCMV 和其他沙粒病毒的几种毒株，包括拉沙热病毒 (LFV)、Oliveros 和 Mobala，与纯化的 α-DG 蛋白结合。可溶性 α-DG 可以阻断 LCMV 和 LFV 感染。携带编码 DG 基因无效突变的细胞对 LCMV 感染有抵抗力，并且无效突变细胞中 DC 表达的重建恢复了对 LCMV 感染的易感性。因此，α-DG 是 LCMV 和 LFV 的细胞受体。
A peripheral membrane protein that is interactive with Lymphocytic choriomeningitis virus (LCMV) was purified from cells permissive to infection. Tryptic peptides from this protein were determined to be alpha-dystroglycan (alpha-DG). Several strains of LCMV and other arenaviruses, including Lassa fever virus (LFV), Oliveros, and Mobala, bound to purified alpha-DG protein. Soluble alpha-DG blocked both LCMV and LFV infection. Cells bearing a null mutation of the gene encoding DG were resistant to LCMV infection, and reconstitution of DC expression in null mutant cells restored susceptibility to LCMV infection. Thus, alpha-DG is a cellular receptor for both LCMV and LFV.