Intermittent access to beer promotes binge-like drinking in adolescent but not adult Wistar rats
Intermittent access to beer promotes binge-like drinking in adolescent but not adult Wistar rats
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DOI：
10.1016/j.alcohol.2009.02.005
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发表时间：
2009-06-01
期刊：
影响因子：
2.3
通讯作者：
McGregor, Iain S.
中科院分区：
文献类型：
--
作者：
Hargreaves, Garth A.;Monds, Lauren;McGregor, Iain S.
青少年比成年人更容易酗酒，并且在长期酗酒后更容易受到大脑变化的影响。我们在啮齿动物模型中研究了青少年过度饮酒的可能性，在该模型中，成年和青少年雄性Wistar大鼠服用啤酒（酒精含量为4.44% /vol）。实验1从出生后28-96天开始，对群养大鼠随意饮用啤酒和水的情况进行了跟踪。大鼠在青春期平均摄入7.8 g/kg/天的乙醇（PND 34-55），在成年期逐渐下降到3.9 g/kg/天的较低水平（PND 56-93）。在实验2中，在定制的“舔水计”装置中，每天给青春期大鼠（PND 29+）和成年大鼠（PND w57+）喝2杯啤酒，持续75天。每3天提供1天啤酒（“间歇”组）或每天提供啤酒（“每日”组）。相对于体重，在这些有限的访问时段，青春期大鼠比成年大鼠消耗了更多的啤酒。间歇性接触的青少年比每天接触的青少年消耗更多，这是一种“狂欢”式的效应，在成人群体中没有观察到，并且在成年后消失。每天或间歇性饮酒3个月后，对啤酒和蔗糖的偏好进行了评估。先前保持间歇性计划的大鼠相对于3%蔗糖显示出更高的啤酒偏好，但只有在禁欲2天后进行测试时。在实验3中，给青春期大鼠（PND 30-37）和成年大鼠（PND 58-65）喝啤酒20分钟，并评估它们的血液酒精浓度（BACs）。青少年组比成年人消耗更多的酒精，并且显示出更高的BACS，这是典型的人类“狂欢”饮酒（80毫克/分升）。尽管如此，在两个年龄组中，血液酒精浓度和啤酒摄入量之间的相关性是相似的。综上所述，这些结果表明，间歇性饮酒本身似乎对饮酒动机有微妙的持久影响。研究结果支持用啤酒溶液来模拟青春期大鼠的狂饮模式。(C) 2009爱思唯尔公司版权所有。
Teenagers are more likely than adults to engage in binge drinking and could be more vulnerable to long-term brain changes following alcohol abuse. We investigated the possibility of excessive adolescent drinking in a rodent model in which beer (4.44% ethanol vol/vol) is presented to adult and adolescent male Wistar rats. Experiment I tracked ad libitum beer and water consumption in group-housed rats from postnatal day (PND) 28-96. Rats consumed an average of 7.8 g/kg/day of ethanol during adolescence (PND 34-55) and this gradually declined to a lower level of intake in adulthood (PND 56-93) of 3.9 g/kg/day. In Experiment 2, beer was made available to both adolescent (PND 29+) and adult (PND w57+) rats for 2 It each day in a custom-built "lickometer" apparatus over 75 days. Access to beer was provided either I day out of every 3 ("intermittent" groups) or every day ("daily" groups). Relative to body weight, adolescent rats consumed more beer than adult rats in these limited access sessions. Adolescents with intermittent access consumed more than adolescents with daily access, a "binge"-like effect that was not observed in adult groups and that disappeared in adulthood. After 3 months of daily or intermittent alcohol consumption, the preference for beer versus sucrose was assessed. Rats previously kept under an intermittent schedule displayed a higher preference for beer relative to 3% sucrose, but only when testing occurred after 2 days of abstinence. In Experiment 3, adolescent (PND 30-37) and adult (PND 58-65) rats were given 20-min access to beer and their blood alcohol concentrations (BACs) were assessed. Adolescent groups consumed more alcohol than adults and showed higher BACS that were typical of human "binge" drinking (> 80 mg/dL). Despite this, the correlation between BAC and beer intake was similar in both age groups. Together these results show that the intermittent presentation of alcohol itself appears to have subtle long-lasting effects on the motivation to consume alcohol. The findings support the use of beer solutions in modeling binge-like patterns of human alcohol consumption in adolescent rats. (C) 2009 Elsevier Inc. All rights reserved.