Eco-Corona vs Protein Corona: Effects of Humic Substances on Corona Formation and Nanoplastic Particle Toxicity in Daphnia magna
Eco-Corona vs Protein Corona: Effects of Humic Substances on Corona Formation and Nanoplastic Particle Toxicity in Daphnia magna
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DOI：
10.1021/acs.est.0c00615
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发表时间：
2020-07-07
影响因子：
11.4
通讯作者：
Guo, Liang-Hong
中科院分区：
文献类型：
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作者：
Fadare, Oluniyi O.;Wan, Bin;Guo, Liang-Hong
尽管有许多关于纳米塑料颗粒（NPP）对水生无脊椎动物的毒性的研究，但腐殖质（HS）等生态成分的影响常常被忽视。在我们的研究中，大型溞被用来评估三种HS：天然有机物（NOM）、黄腐酸（FA）和腐殖酸（HA）对NPP毒性和电晕形成的影响。所有HS在环境相关浓度下均降低了核电厂的急性毒性。接触 NPP 7 天后，所有与解毒、氧化应激和内分泌活动相关的基因都会上调。 NOM 或 HA 的存在导致基因表达减弱，而 FA 则观察到所有基因的上调显着更高。 FA 的存在导致 D. magna 培养基（eco-corona，EC）和匀浆（蛋白质 corona，PC）中 NPP 的蛋白质吸附增加，而 FA 存在时吸附较少。 EC 中鉴定出的高丰度蛋白质涉及免疫防御、细胞维持和抗捕食者反应，而 PC 中的蛋白质负责脂质运输、抗氧化作用和雌激素介导。我们的研究结果揭示了HS对核电厂毒性的关键影响，并为未来的研究提供了分析和概念基础。
Despite many studies on the toxicity of nanoplastic particles (NPPs) to aquatic invertebrates, the effects of ecological constituents such as humic substances (HSs) are often neglected. In our study, Daphnia magna was used to evaluate the effects of three HSs, natural organic matter (NOM), fulvic acid (FA), and humic acid (HA), on NPP toxicity and corona formation. Acute toxicities of NPPs were reduced by all HSs at environmentally relevant concentrations. NPPs elicited the upregulation of all genes related to detoxification, oxidative stress, and endocrine activity after 7 days of exposure. The presence of NOM or HA resulted in the mitigation of gene expression, whereas significantly higher upregulation of all of the genes was observed with FA. The presence of FA led to increased protein adsorption on NPPs in D. magna culture medium (eco-corona, EC) and homogenates (protein corona, PC), while there was less adsorption in the presence of FA. The highly abundant proteins identified in EC are involved in immune defense, cell maintenance, and antipredator response, while those in PC are responsible for lipid transport, antioxidant effects, and estrogen mediation. Our findings revealed the key influence of HSs on the toxicity of NPPs and provide an analytical and conceptual foundation for future study.