Characterization and gonadal expression of FOXL2 relative to Cyp19a genes in spotted scat Scatophagus argus
Characterization and gonadal expression of FOXL2 relative to Cyp19a genes in spotted scat Scatophagus argus
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斑点鱼粪中 FOXL2 相对于 Cyp19a 基因的特征和性腺表达
DOI：
10.1016/j.gene.2014.12.060
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发表时间：
2015-04-25
期刊：
影响因子：
3.5
通讯作者：
Zhang, Junbin
中科院分区：
文献类型：
--
作者：
Liu, Huifen;Mu, Xingjiang;Zhang, Junbin
本研究克隆了斑点鱼FOXL2、Cyp19a1a和Cyp19a1b的全长cdna，并分析了它们在性腺发育过程中的表达模式。这三个基因均在卵巢和睾丸中表达，但表现出两性二态性。在性腺发育早期，卵巢中FOXL2的表达高于睾丸。随着性腺发育的继续，FOXL2表达逐渐降低，在成熟阶段达到最低水平。除卵巢卵黄形成早期外，Cyp19a1a和Cyp19alb与FOXL2协同表达。FOXL2、Cyp19a1a和Cyp19a1b的表达主要局限于卵巢颗粒细胞。在睾丸中，FOXL2基因在小管和间质细胞等间质细胞中表达强烈，而Cyp19a1a和Cyp19a1b在整个配子体发生过程中主要在支持细胞中表达。这些结果表明FOXL2在性发育中起着至关重要的作用，并暗示它可能调节银条螺中Cyp19a1a和Cyp19a1b的表达。(C) 2014 Elsevier B.V.版权所有
In the present study, we cloned the full-length cDNAs of FOXL2, Cyp19a1a and Cyp19a1b and analyzed their expression patterns during gonadal development in spotted scat, Scatophagus argus. All three genes were expressed in ovaries and testes but showed sexual dimorphism. At early stages of gonadal development, the expression of FOXL2 in ovaries was higher than testes. FOXL2 expression deceased gradually as gonadal development continued, and reached the lowest level at the mature stage. Cyp19a1a and Cyp19alb were expressed coordinately with FOXL2, except at the early vitellogenic stage in the ovary. The expression of FOXL2, Cyp19a1a and Cyp19a1b was mainly localized in granulosa cells of ovaries. In S. argus testes, strong expression of FOXL2 gene was observed in the interstitial cells including tubules and Leydig cells, while Cyp19a1a and Cyp19a1b were mainly expressed in Sertoli cells throughout gametogenesis. These results show that FOXL2 plays an essential role in sexual development, and imply that it may regulate Cyp19a1a and Cyp19a1b expression in S. argus. (C) 2014 Elsevier B.V. All rights reserved.