Antimycotic-Antibiotic Amphotericin B Promotes Influenza Virus Replication in Cell Culture
Antimycotic-Antibiotic Amphotericin B Promotes Influenza Virus Replication in Cell Culture
复制标题
DOI：
10.1128/jvi.00169-11
复制
发表时间：
2011-11-01
影响因子：
5.4
通讯作者：
Egorov, Andrej
中科院分区：
文献类型：
--
作者：
Roethl, Elisabeth;Gassner, Manuela;Egorov, Andrej
一般来说，抗生素不被列为抑制或支持流感病毒复制的物质。我们在这里描述了多烯抗生素两性霉素B （AmB）对流感病毒在Vero细胞中的生长的增强作用。我们发现，在培养基中添加AmB可以显著提高临床样本中甲型和乙型流感病毒的分离率。我们证明了AmB促进病毒的摄取和病毒颗粒的内吞加工。这种作用对Vero和人类鼻上皮细胞是特异性的，而在Madin-Darby犬肾细胞中未观察到。AmB的作用是亚型特异性的，对人类季节性流感毒株的作用更为突出，而对H5N1人类病毒的作用则不存在。amb增强效应似乎仅仅是由于病毒血凝素的功能。我们的研究结果表明，使用AmB可能促进流感病毒在Vero细胞中的分离和产生。
In general, antibiotics are not rated as substances that inhibit or support influenza virus replication. We describe here the enhancing effect of the polyene antibiotic amphotericin B (AmB) on influenza virus growth in Vero cells. We show that isolation rates of influenza A and B viruses from clinical samples can be dramatically enhanced by adding AmB to the culture medium. We demonstrate that AmB promotes the viral uptake and endocytic processing of the virus particles. This effect is specific for Vero and human nasal epithelial cells and was not observed in Madin-Darby canine kidney cells. The effect of AmB was subtype specific and more prominent for human seasonal influenza strains but absent for H5N1 human viruses. The AmB-enhancing effect seemed to be solely due to the viral hemagglutinin function. Our results indicate that the use of AmB may facilitate influenza virus isolation and production in Vero cells.