Humanin skeletal muscle protein levels increase after resistance training in men with impaired glucose metabolism.
Humanin skeletal muscle protein levels increase after resistance training in men with impaired glucose metabolism.
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DOI：
10.14814/phy2.13063
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发表时间：
2016-12
影响因子：
2.5
通讯作者：
Sundberg CJ
中科院分区：
文献类型：
--
作者：
Gidlund EK;von Walden F;Venojärvi M;Risérus U;Heinonen OJ;Norrbom J;Sundberg CJ
护脑素 (HN) 是一种线粒体编码和分泌的肽，与葡萄糖代谢和组织保护机制有关。骨骼肌 HN 基因或蛋白质表达是否受运动影响仍不清楚。在这项干预研究中，我们首次表明，糖尿病前期患者经过 12 周的阻力训练后，人体骨骼肌中的 HN 蛋白水平有所增加。招募了血糖调节受损 (IGR) 的男性受试者 (n = 55)，并随机分配到阻力训练组、越野行走组或对照组。运动干预每周进行 3 次，持续 12 周，并在干预期间逐渐增加强度。在干预前后采集股外侧肌活检和静脉血样本。分析了 HN 的骨骼肌和血清蛋白水平，以及线粒体编码基因 MT-RNR2 的骨骼肌基因表达，其中包含 HN 的开放阅读框。为了阐明线粒体训练适应，测量了线粒体 DNA、核 DNA 以及柠檬酸合酶。经过 12 周的阻力训练后，骨骼肌 HN 蛋白水平增加了 35%。在任何干预组中，血清中的护脑素蛋白水平都没有变化。抗阻训练组血清中的护脑素水平与 2 小时葡萄糖负荷测试的改善之间存在显着相关性。糖尿病前期男性进行定期阻力训练后骨骼肌中 HN 蛋白水平的增加可能表明 HN 在葡萄糖代谢调节中的作用。鉴于运动对 2 型糖尿病的预防作用，HN 作为线粒体衍生肽和运动反应性线粒体因子的作用值得进一步研究。
Humanin (HN) is a mitochondrially encoded and secreted peptide linked to glucose metabolism and tissue protecting mechanisms. Whether skeletal muscle HN gene or protein expression is influenced by exercise remains unknown. In this intervention study we show, for the first time, that HN protein levels increase in human skeletal muscle following 12 weeks of resistance training in persons with prediabetes. Male subjects (n = 55) with impaired glucose regulation (IGR) were recruited and randomly assigned to resistance training, Nordic walking or a control group. The exercise interventions were performed three times per week for 12 weeks with progressively increased intensity during the intervention period. Biopsies from the vastus lateralis muscle and venous blood samples were taken before and after the intervention. Skeletal muscle and serum protein levels of HN were analyzed as well as skeletal muscle gene expression of the mitochondrially encoded gene MT‐RNR2, containing the open reading frame for HN. To elucidate mitochondrial training adaptation, mtDNA, and nuclear DNA as well as Citrate synthase were measured. Skeletal muscle HN protein levels increased by 35% after 12 weeks of resistance training. No change in humanin protein levels was seen in serum in any of the intervention groups. There was a significant correlation between humanin levels in serum and the improvements in the 2 h glucose loading test in the resistance training group. The increase in HN protein levels in skeletal muscle after regular resistance training in prediabetic males may suggest a role for HN in the regulation of glucose metabolism. Given the preventative effect of exercise on diabetes type 2, the role of HN as a mitochondrially derived peptide and an exercise‐responsive mitokine warrants further investigation.