ACE1, A COPPER-DEPENDENT TRANSCRIPTION FACTOR, ACTIVATES EXPRESSION OF THE YEAST COPPER, ZINC SUPEROXIDE-DISMUTASE GENE
ACE1, A COPPER-DEPENDENT TRANSCRIPTION FACTOR, ACTIVATES EXPRESSION OF THE YEAST COPPER, ZINC SUPEROXIDE-DISMUTASE GENE
复制标题
DOI：
10.1073/pnas.88.19.8558
复制
发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
VALENTINE, JS
中科院分区：
文献类型：
--
作者：
GRALLA, EB;THIELE, DJ;VALENTINE, JS
铜锌超氧化物歧化酶（SOD1基因产物）（superoxide:superoxide oxidoreductase, EC 1.15.1.1）是一种具有防止氧毒性作用的含铜酶。在酿酒酵母中，铜水平对SOD1的表达水平起一定的控制作用。我们发现，负责诱导酵母金属硫蛋白（CUP1）响应铜的ACE1转录激活蛋白也控制SOD1对铜的响应。DNase I保护和甲基化干扰实验表明，SOD1启动子区域存在ACE1的单一结合位点，并且与CUP1启动子中一个高亲和力的ACE1结合位点高度同源。这种dna -蛋白质相互作用的功能重要性可以通过以下事实证明：(i)铜诱导SOD1 mRNA不会发生在缺乏ACE1的菌株中，（ii）它不会发生在含有缺乏ACE1功能结合位点的基因工程SOD1启动子的菌株中。
Copper, zinc superoxide dismutase (SOD1 gene product) (superoxide:superoxide oxidoreductase, EC 1.15.1.1) is a copper-containing enzyme that functions to prevent oxygen toxicity. In the yeast Saccharomyces cerevisiae, copper levels exert some control over the level of SOD1 expression. We show that the ACE1 transcriptional activator protein, which is responsible for the induction of yeast metallothionein (CUP1) in response to copper, also controls the SOD1 response to copper. A single binding site for ACE1 is present in the SOD1 promoter region, as demonstrated by DNase I protection and methylation interference experiments, and is highly homologous to a high-affinity ACE1 binding site in the CUP1 promoter. The functional importance of this DNA-protein interaction is demonstrated by the facts that (i) copper induction of SOD1 mRNA does not occur in a strain lacking ACE1 and (ii) it does not occur in a strain containing a genetically engineered SOD1 promoter that lacks a functional ACE1 binding site.