Assessing the fate of brown trout (Salmo trutta) environmental DNA in a natural stream using a sensitive and specific dual-labelled probe
Assessing the fate of brown trout (Salmo trutta) environmental DNA in a natural stream using a sensitive and specific dual-labelled probe
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DOI：
10.1016/j.scitotenv.2018.11.247
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发表时间：
2019-03-10
影响因子：
9.8
通讯作者：
Brinkmann, Markus
中科院分区：
文献类型：
--
作者：
Deutschmann, Bjoern;Mueller, Anne-Kathrin;Brinkmann, Markus
水环境中的环境DNA(EDNA)分析已成为综合监测战略中诊断生态状况的一种很有前途的工具，并可能在欧洲水框架指令(WFD)和其他立法的背景下用于推导特定应激源的指标。尽管在生态系统组成和内陆水域入侵/稀有物种的检测方面，许多研究已经在EDNA的未来利用方面取得了重大进展，但关于EDNA在自然环境条件下的运输和命运仍有许多未知之处。我们设计了一种特异的双标记探针来检测褐鲑鱼(Salmo trutta，L.)埃德娜并用探测器描述了埃德娜从水产养殖场被释放到德国韦赫巴赫低山溪流中的命运。该探针被证明是专门针对褐鲑鱼的，因为池塘里养着彩虹鲑鱼(Oncorhynchus Mykiss)，检测结果不呈阳性。尽管我们观察到三个含有不同褐鲑鱼数量的池塘的EDNA信号强度不同，但生物量(kg/L)与EDNA数量之间没有显著的相关性。我们的结果表明，褐鲑鱼DNA的释放可能具有生活期和/或年龄依赖性。水产养殖设施的流出物是EDNA含量较高的来源，这导致该设施下游的褐鲑鱼Edna数量最多。尽管在Wehebach河中自然出现了褐鲑鱼，但正如德国北莱茵-威斯特伐利亚州当局进行的生态调查和个人观察所显示的那样，我们观察到在水产养殖下游的第一个1.5公里内，Wehebach河中Edna的相对丰度显著下降。我们的结果表明，在自然条件下，由于稀释和降解过程，自来水中EDNA的浓度迅速下降，这可能对EDNA在环境研究中的应用具有重要意义。(C)2018爱思唯尔B.V.保留所有权利。
Environmental DNA (eDNA) analysis in the aquatic environment has emerged as a promising tool for diagnosis of the ecological status in comprehensive monitoring strategies and might become useful in context of the EuropeanWater Framework Directive (WFD) and other legislations to derive stressor-specific indicators. Despite many studies having made significant progress for the future use of eDNA in terms of ecosystem composition and detection of invasive/rare species in inland waters, much remains unknown about the transport and fate of eDNA under natural environmental conditions. We designed a specific dual-labelled probe to detect brown trout (Salmo trutta, L.) eDNA and used the probe to describe the fate of eDNA released from an aquaculture facility into the low mountain range stream Wehebach, Germany. The probe was shown to be specific to brown trout, as ponds housing rainbow trout (Oncorhynchus mykiss) did not test positive. Even though we observed different strengths of eDNA signals for three ponds containing different brown trout quantities, no significant correlation was found between biomass (kg/L) and eDNA quantity. Our results indicate that the release of DNA from brown trout might be life stage and/or age-dependent. The effluents of the aquaculture facility were a source of high levels of eDNA which resulted in the greatest abundance of brown trout eDNA directly downstream of the facility. Despite the natural occurrence of brown trout in the Wehebach, as shown by ecological investigations conducted by authorities of the federal state of North Rhine-Westphalia (Germany) and personal observations, we observed a significant decrease of relative abundance of eDNA in the Wehebach within the first 1.5 km downstream of the Aquaculture. Our results suggest that concentrations of eDNA in running waters rapidly decrease under natural conditions due to dilution and degradation processes, which might have important implications for the utility of eDNA in environmental research. (C) 2018 Elsevier B.V. All rights reserved.