Plastidial glycolysis in developing Arabidopsis embryos
Plastidial glycolysis in developing Arabidopsis embryos
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DOI：
10.1111/j.1469-8137.2009.03113.x
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发表时间：
2010-02-01
期刊：
影响因子：
9.4
通讯作者：
Smith, Alison M.
中科院分区：
文献类型：
--
作者：
Andriotis, Vasilios M. E.;Kruger, Nicholas J.;Smith, Alison M.
在油籽胚胎发育过程中，来自蔗糖的碳被用于质体中的脂肪酸合成。研究了质体糖酵解在拟南芥胚油积累中的作用。鉴定了编码烯醇酶（ENO）和磷酸甘油变位酶（PGlyM）的基因，并通过重组和绿色荧光蛋白（GFP）融合蛋白的表达确定了活性和亚细胞位置。缺乏推定质体亚型的突变拟南芥植物的亚型组成和胚油含量进行了表征。在发育中的胚胎中，ENO1和ENO2分别占大部分或全部质体和细胞质ENO活性，PGLYM1占大部分或全部质体PGlyM活性。 eno1和pglym1突变体，其中质体ENO和PGlyM活性不可检测，在成熟时具有野生型量的种子油。结论是，虽然发育中的拟南芥胚胎的质体具有执行糖酵解途径的较低部分的能力，但单独的胞质糖酵解途径足以支持来自3-磷酸甘油酸的通量 转化为石油生产所需的磷酸烯醇丙酮酸。结果强调了易位子对于油生产的重要性，易位子促进细胞质和质体基质之间糖酵解中间体的交换。
During oilseed embryo development, carbon from sucrose is utilized for fatty acid synthesis in the plastid. The role of plastidial glycolysis in Arabidopsis embryo oil accumulation was investigated.Genes encoding enolases (ENO) and phosphoglyceromutases (PGlyM) were identified, and activities and subcellular locations were established by expression of recombinant and green fluorescent protein (GFP)-fusion proteins. Mutant Arabidopsis plants lacking putative plastidial isoforms were characterized with respect to isoform composition and embryo oil content.In the developing embryo, ENO1 and ENO2 account for most or all of the plastidial and cytosolic ENO activity, respectively, and PGLYM1 accounts for most or all of the plastidial PGlyM activity. The eno1 and pglym1 mutants, in which plastidic ENO and PGlyM activities were undetectable, had wild-type amounts of seed oil at maturity.It is concluded that although plastids of developing Arabidopsis embryos have the capacity to carry out the lower part of the glycolytic pathway, the cytosolic glycolytic pathway alone is sufficient to support the flux from 3-phosphoglycerate to phosphoenolpyruvate required for oil production. The results highlight the importance for oil production of translocators that facilitate interchange of glycolytic intermediates between the cytosol and the plastid stroma.