Visualization of the endocytic pathway in the filamentous fungus Aspergillus oryzae using an EGFP-fused plasma membrane protein
Visualization of the endocytic pathway in the filamentous fungus Aspergillus oryzae using an EGFP-fused plasma membrane protein
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DOI：
10.1016/j.bbrc.2005.12.077
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发表时间：
2006-02-17
影响因子：
3.1
通讯作者：
Kitamoto, K
中科院分区：
文献类型：
--
作者：
Higuchi, Y;Nakahama, T;Kitamoto, K
内吞作用是细胞活动的重要过程。然而，在丝状真菌中，内吞作用的存在至今仍是难以捉摸的。在这项研究中，我们使用AoUapC-EGFP，一个假定的尿酸-黄嘌呤通透酶与增强型绿色荧光蛋白（EGFP）的融合蛋白在曲霉，以检查是否发生内吞过程。在培养基中加入铵后，融合蛋白从质膜内化。AoUapC-EGFP的内化被叠氮化钠、冷和细胞松弛素A处理完全阻断，表明内化具有内吞作用的一般特征。这些结果表明在丝状真菌中内吞作用的发生。此外，我们发现内吞作用诱导后，内体区室出现，并以微管依赖的方式移动。(c)2005年爱思唯尔公司All rights reserved.
Endocytosis is an important process for cellular activities. However, in filamentous fungi, the existence of endocytosis has been so far elusive. In this study, we used AoUapC-EGFP, the fusion protein of a putative Uric acid-xanthine permease with enhanced green fluorescent protein (EGFP) in Aspergillus oryzae, to examine whether the endocytic process occurs or not. Upon the addition of ammonium into the medium the fusion protein was internalized from the plasma membrane. The internalization of AoUapC-EGFP was completely blocked by sodium azide, cold, and cytochalasin A treatments, suggesting that the internalization possesses the general features of endocytosis. These results demonstrate the occurrence of endocytosis in filamentous fungi. Moreover, we discovered that the endosomal compartments appeared upon the induction of endocytosis and moved in a microtubule-dependent manner. (c) 2005 Elsevier Inc. All rights reserved.