gamma-Aminobutyric acid-activated 36Cl- influx: a functional in vitro assay for CNS gamma-aminobutyric acid receptors of insects.
gamma-Aminobutyric acid-activated 36Cl- influx: a functional in vitro assay for CNS gamma-aminobutyric acid receptors of insects.
复制标题
γ-氨基丁酸激活的 36Cl- 流入：昆虫 CNS γ-氨基丁酸受体的功能性体外测定。
DOI：
10.1111/j.1471-4159.1987.tb13144.x
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发表时间：
1987
影响因子：
4.7
通讯作者：
Eldefrawi,ME
中科院分区：
文献类型：
--
作者：
Wafford,KA;Sattelle,DB;Abalis,I;Eldefrawi,AT;Eldefrawi,ME
本文研究了氨基丁酸(γ)对美洲大蠊神经索膜微囊吸收~(36)Cl的影响。加入1μ的MGABA(4-S孵育后，快速猝灭)，可刺激~(36)Cl-的内流，比对照高出75%。这种刺激可被印防己毒素(100μM)减弱，但不受荷包牡丹碱(100μM)的显著影响。~(36)Cl-内流实验结果与放射性标记配体结合实验和电生理实验结果一致。该方法是一种昆虫中枢神经系统GABA受体的体外功能性分析方法。
The effects of γaminobutyric acid (GABA) on the uptake of36Cl into a membrane microsac preparation from isolated nerve cords of the cockroachPeriplaneta americanawas studied. On addition of 1 μMGABA (after 4‐s incubation, then rapid quenching) the influx of36Cl‐was stimulated to a level 75% above that of the control value. This stimulation was reduced by picrotoxin (100 μM), but was not significantly affected by bicuculline (100 μM)Results of36Cl‐influx experiments are in agreement with data obtained from radiolabelled ligand binding assays and electrophysiological investigations on the same tissue. The method described represents a functional in vitro assay for CNS GABA receptors of insects.