A Bright Light to Reveal Mobility: Single Quantum Dot Tracking Reveals Membrane Dynamics and Cellular Mechanisms.
A Bright Light to Reveal Mobility: Single Quantum Dot Tracking Reveals Membrane Dynamics and Cellular Mechanisms.
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DOI：
10.1021/jz401071g
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发表时间：
2013-08-08
期刊：
影响因子：
--
通讯作者：
Rosenthal SJ
中科院分区：
文献类型：
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作者：
Chang JC;Rosenthal SJ
这个观点描述了单量子点技术的最新进展，重点是它们在探索膜动力学和细胞机制方面的应用。在这些情况下，传统的群体测量（例如光漂白后的荧光恢复）仅产生整体或大量分子集合的平均值，其中缺少单个蛋白质和载体的行为。近年来，单量子点成像方法作为单分子荧光技术的一个子类别被引入，以实时揭示单一蛋白质/载体动力学。使用单量子点的主要优点之一是高信噪比，这源于其独特的光物理特性，例如极高的摩尔消光系数和大的有效斯托克斯位移。除了简要概述单量子点成像技术的原理外，我们还重点介绍了最近的发现并讨论了该领域的未来方向。
This perspective describes recent progress in single quantum dot techniques, with an emphasis on their applications in exploring membrane dynamics and cellular mechanisms. In these cases, conventional population measurements, such as fluorescence recovery after photobleaching, yield only a mean value on an ensemble or bulk collection of molecules, where the behavior of individual proteins and vehicles is missing. In recent years, the single quantum dot imaging approach has been introduced as a sub-category of single molecule fluorescent techniques to reveal single protein/vehicle dynamics in real-time. One of the major advantages of using single quantum dots is the high signal-to-noise ratio originating from their unique photophysical properties such as extraordinarily high molar extinction coefficients and large effective Stokes shifts. In addition to a brief overview on the principle of single quantum dot imaging techniques, we highlight recent discoveries and discuss future directions in the field.