Identification and quantification of phytochelatins in roots of rice to long-term exposure: evidence of individual role on arsenic accumulation and translocation.
Identification and quantification of phytochelatins in roots of rice to long-term exposure: evidence of individual role on arsenic accumulation and translocation.
复制标题
DOI：
10.1093/jxb/eru018
复制
发表时间：
2014-04
影响因子：
6.9
通讯作者：
Feldmann J
中科院分区：
文献类型：
--
作者：
Batista BL;Nigar M;Mestrot A;Rocha BA;Barbosa Júnior F;Price AH;Raab A;Feldmann J
在同一土壤中，六种水稻分别暴露在低砷和高砷环境中。它们表达植物螯合素的个体反应与水稻对无机砷的吸收、运输和积累有关。水稻倾向于以甲基化砷（o-As）和无机砷（i-As）的形式吸收砷。植物利用i-As外排系统和植物螯合素（PCs）的产生来复合物i-As来保护自己。我们的研究重点是通过HPLC-ICP-MS/ESI-MS鉴定和定量植物螯合素，并将它们与砷暴露相关的几个变量联系起来。研究了6个水稻品种（Italica Carolina、Dom Sofid、9524、Kitrana 508、YRL-1和Lemont）在低、高浓度i-As环境下的GSH、11 PCs和As-PC复合物与根、芽和籽粒中总as、i-As和o-As含量的差异。只有Dom Sofid、Kitrana 508和9524被发现即使暴露在低水平的砷中也会产生更高水平的pc。根系中pc仅与i-As相关（r=0.884, P <0.001）。但与根粒间（r= -0.739, P <0.05）和茎粒间（r= -0.541, P <0.05）的As传递因子（TF）呈显著负相关，表明这些肽有助于捕获根中的i-As，而不是o-As，从而降低了籽粒中的i-As。意大利卡罗莱纳在高暴露后减少了谷物中的i-As，其中一些特定的pc在这种减少中起着特殊的作用。在莱蒙特，暴露于高水平的i-As并没有导致谷物中更高的i-As水平，pc和硫醇也没有显著增加。最后，Kitrana 508和Dom Sofid在高砷处理下的高PCs产量与籽粒中i-As的减少无关，这表明其他机制，如As - pc的释放和运输似乎在决定这些品种籽粒As方面很重要。
Six varieties of rice were exposed to low and high levels of arsenic in the same soil. Their individual responses of expressing phytochelatins have been correlated to inorganic arsenic uptake, transport, and accumulation in the rice grain. Rice has the predilection to take up arsenic in the form of methylated arsenic (o-As) and inorganic arsenic species (i-As). Plants defend themselves using i-As efflux systems and the production of phytochelatins (PCs) to complex i-As. Our study focused on the identification and quantification of phytochelatins by HPLC-ICP-MS/ESI-MS, relating them to the several variables linked to As exposure. GSH, 11 PCs, and As–PC complexes from the roots of six rice cultivars (Italica Carolina, Dom Sofid, 9524, Kitrana 508, YRL-1, and Lemont) exposed to low and high levels of i-As were compared with total, i-As, and o-As in roots, shoots, and grains. Only Dom Sofid, Kitrana 508, and 9524 were found to produce higher levels of PCs even when exposed to low levels of As. PCs were only correlated to i-As in the roots (r=0.884, P <0.001). However, significant negative correlations to As transfer factors (TF) roots–grains (r= –0.739, P <0.05) and shoots–grains (r= –0.541, P <0.05), suggested that these peptides help in trapping i-As but not o-As in the roots, reducing grains’ i-As. Italica Carolina reduced i-As in grains after high exposure, where some specific PCs had a special role in this reduction. In Lemont, exposure to elevated levels of i-As did not result in higher i-As levels in the grains and there were no significant increases in PCs or thiols. Finally, the high production of PCs in Kitrana 508 and Dom Sofid in response to high As treatment did not relate to a reduction of i-As in grains, suggesting that other mechanisms such as As–PC release and transport seems to be important in determining grain As in these cultivars.
登录
查看更多内容
影响因子：
7.4
作者：
Cobbett, CS
通讯作者：
Cobbett, CS
影响因子：
6.9
作者：
MEHARG, AA;MACNAIR, MR
通讯作者：
MACNAIR, MR
影响因子：
7.4
作者：
Liu, Wen-Ju;Wood, B. Alan;Feldmann, Joerg
通讯作者：
Feldmann, Joerg
影响因子：
7.4
作者：
Amayo, Kenneth O.;Petursdottir, Asta;Feldmann, Joerg
通讯作者：
Feldmann, Joerg
影响因子：
9.4
作者：
Chen, Z;Zhu, YG;Meharg, AA
通讯作者：
Meharg, AA