Endomembrane and Golgi traffic in plant cells.
Endomembrane and Golgi traffic in plant cells.
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DOI：
10.1016/b978-0-12-417164-0.00005-7
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发表时间：
2013
影响因子：
--
通讯作者：
Brandizzi, Federica
中科院分区：
文献类型：
--
作者：
Stefano, Giovanni;Osterrieder, Anne;Hawes, Chris;Brandizzi, Federica
叶表皮细胞是研究植物分泌途径的理想样本，因为它相对容易用荧光蛋白标记，并在体内可视化该途径的各种细胞器。许多技术可以用来鉴定和研究膜细胞器内的蛋白质，并研究它们的动力学和相互作用。在这里，我们讨论了最常用的在拟南芥和烟叶中表达蛋白质的方法，使用突变体筛选来识别运输蛋白质，以及使用两种体内技术，光漂白后的荧光恢复和Förster共振能量转移，来研究植物细胞中的蛋白质动力学。
Leaf epidermal cells make ideal specimens for the investigation of the plant secretory pathway in that it is relatively easy to tag with fluorescent proteins and visualize in vivo the various organelles of the pathway. A number of techniques can be employed to identify and study proteins within the endomembrane organelles and to study their dynamics and interactions. Here, we discuss the most commonly used approaches to express proteins within arabidopsis and tobacco leaves, the use of mutant screens to identify trafficking proteins, and the use of two in vivo techniques, Fluorescence recovery after photobleaching and Förster resonance energy transfer, to study protein dynamics in plant cells.