Switch from capsid protein import to adenovirus assembly by cleavage of nuclear transport signals.
Switch from capsid protein import to adenovirus assembly by cleavage of nuclear transport signals.
复制标题
通过裂解核转运信号从衣壳蛋白输入转变为腺病毒组装。
DOI：
10.1093/emboj/cdg614
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Gerace,Larry
中科院分区：
文献类型：
--
作者：
Wodrich,Harald;Guan,Tinglu;Cingolani,Gino;VonSeggern,Dan;Nemerow,Glen;Gerace,Larry
腺病毒的复制和组装发生在感染细胞的细胞核中，需要所有病毒结构蛋白的核输入。在这份报告中，我们表明，核进口的主要衣壳蛋白，六邻体，是介导的蛋白VI，结构蛋白位于下方的12个顶点的腺病毒衣壳。我们的数据表明，蛋白VI穿梭于细胞核和细胞质之间，并且它通过importin α/β依赖性机制将六邻体连接到核输入机器。蛋白VI的关键核输入和输出信号位于短的C末端区段，其在病毒成熟期间被蛋白水解去除。这些C末端转运信号的去除似乎触发了蛋白VI的功能转变，从支持六邻体核输入的作用到病毒组装中的结构作用。
Replication and assembly of adenovirus occurs in the nucleus of infected cells, requiring the nuclear import of all viral structural proteins. In this report we show that nuclear import of the major capsid protein, hexon, is mediated by protein VI, a structural protein located underneath the 12 vertices of the adenoviral capsid. Our data indicate that protein VI shuttles between the nucleus and the cytoplasm and that it links hexon to the nuclear import machinery via an importin α/β‐dependent mechanism. Key nuclear import and export signals of protein VI are located in a short C‐terminal segment, which is proteolytically removed during virus maturation. The removal of these C‐terminal transport signals appears to trigger a functional transition in protein VI, from a role in supporting hexon nuclear import to a structural role in virus assembly.