Geographical distribution of Anopheles stephensi in eastern Ethiopia
Geographical distribution of Anopheles stephensi in eastern Ethiopia
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DOI：
10.1186/s13071-020-3904-y
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发表时间：
2020-01-20
影响因子：
3.2
通讯作者：
Carter, Tamar E.
中科院分区：
文献类型：
--
作者：
Balkew, Meshesha;Mumba, Peter;Carter, Tamar E.
背景最近在埃塞俄比亚和非洲之角其他地区发现的南亚疟疾媒介斯氏按蚊引起了人们对其对疟疾传播的潜在影响的关注。我们在此报告了在埃塞俄比亚东部使用形态和分子方法对这种物种进行鉴定的调查结果。方法在埃塞俄比亚东部10个地点采集成虫和幼虫/蛹，用标准形态检索法和遗传分析鉴定按蚊标本。结果共分离出2231株经形态鉴定的An。Stephensi)。一种结合了PCR终点分析和内部转录间隔区2(ITS2)和细胞色素C氧化酶亚单位1(COX1)基因座部分序列的分子方法证实了AN的身份。斯氏杆菌在大多数病例中(119/124)在形态上被鉴定为斯氏杆菌。斯蒂芬西从分子上证实了这一点)。此外，我们还观察到埃及伊蚊的幼虫和幼虫在许多蚊虫体内。斯氏幼虫的栖息地。结论我们的研究结果表明，An.斯氏杆菌广泛分布在埃塞俄比亚东部，并突出表明需要在该国南部、西部和北部以及整个非洲之角进行进一步监测。
Background The recent detection of the South Asian malaria vector Anopheles stephensi in Ethiopia and other regions in the Horn of Africa has raised concerns about its potential impact on malaria transmission. We report here the findings of a survey for this species in eastern Ethiopia using both morphological and molecular methods for species identification. Methods Adult and larval/pupal collections were conducted at ten sites in eastern Ethiopia and Anopheles specimens were identified using standard morphological keys and genetic analysis. Results In total, 2231 morphologically identified An. stephensi were collected. A molecular approach incorporating both PCR endpoint assay and sequencing of portions of the internal transcribed spacer 2 (ITS2) and cytochrome c oxidase subunit 1 (cox1) loci confirmed the identity of the An. stephensi in most cases (119/124 of the morphologically identified An. stephensi confirmed molecularly). Additionally, we observed Aedes aegypti larvae and pupae at many of the An. stephensi larval habitats. Conclusions Our findings show that An. stephensi is widely distributed in eastern Ethiopia and highlight the need for further surveillance in the southern, western and northern parts of the country and throughout the Horn of Africa.