Adaptive mutation: general mutagenesis is not a programmed response to stress but results from rare coamplification of dinB with lac.
Adaptive mutation: general mutagenesis is not a programmed response to stress but results from rare coamplification of dinB with lac.
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适应性突变：一般诱变不是对应激的程序性反应，而是 dinB 与 lac 罕见共扩增的结果。
DOI：
10.1073/pnas.1735464100
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Roth,JohnR
中科院分区：
文献类型：
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作者：
Slechta,ESusan;Bunny,KimL;Kugelberg,Elisabeth;Kofoid,Eric;Andersson,DanI;Roth,JohnR
在特定的遗传系统中，选择刺激突变逆转，并导致全基因组突变(适应性突变)。选择允许带有泄漏等位基因的稀有平板细胞启动克隆，在其中进一步扩增可以提高生长速度。生长和扩增为每个克隆增加了突变靶点，从而增加了逆转的可能性。我们认为，一般的突变只发生在克隆中，其扩增包括邻近的dinB+基因(对于容易出错的DNA聚合酶IV)。因此，突变不是对胁迫的程序化反应，而是少数克隆扩增的副作用；它不是选择对逆转的影响的核心。
In a particular genetic system, selection stimulates reversion of alacmutation and causes genome-wide mutagenesis (adaptive mutation). Selection allows rare plated cells with a duplication of the leakylacallele to initiate clones within which furtherlacamplification improves growth rate. Growth and amplification add mutational targets to each clone and thereby increase the likelihood of reversion. We suggest that general mutagenesis occurs only in clones whoselacamplification includes the nearbydinB+gene (for error-prone DNA polymerase IV). Thus mutagenesis is not a programmed response to stress but a side effect of amplification in a few clones; it is not central to the effect of selection on reversion.