METHODS FOR THE DETERMINATION OF THE CIRCULATING CONCENTRATION OF 25-HYDROXYVITAMIN-D
METHODS FOR THE DETERMINATION OF THE CIRCULATING CONCENTRATION OF 25-HYDROXYVITAMIN-D
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DOI：
10.1016/0955-2863(90)90067-u
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发表时间：
1990-06-01
影响因子：
5.6
通讯作者：
HOLICK, MF
中科院分区：
文献类型：
--
作者：
CHEN, TC;TURNER, AK;HOLICK, MF
25-羟基维生素D2 （25-OH-D2）和25-羟基维生素D3 （25-OH-D3）组合的循环浓度是来自阳光和饮食的维生素D的总和。为了确定其浓度，首先用无水乙醇从血清或血浆中提取25-OH-D (25-OH-D2和/或25-OH-D3)，然后使用与25-OH-D具有高亲和力的血清维生素D结合蛋白进行蛋白质结合试验。为了准确测定没有其他维生素D代谢物的25-OH-D的循环浓度，可以使用硅胶Sep-Pak药筒色谱法。在本文中，我们描述了在我们的实验室中用于测定血浆和血清中25-OH-D的方法。测定维生素D的方法已在本刊上报道过。下一篇文章描述了1,25 -二羟基维生素D的测定方法。
The circulating concentration of the combination of 25-hydroxyvitamin D2 (25-OH-D2) and 25-hydroxyvitamin D3 (25-OH-D3) is a summation of the vitamin D that comes from sunlight and the diet. 1 This measurement is considered to be the best indicator of vitamin D statusfl To determine its concentration, 25-OH-D (25-OH-D2 and/or 25-OH-D3) is first extracted from serum or plasma with absolute ethanol, followed by a protein-binding assay using the serum vitamin D-binding protein which has high affinity for 25-OH-D. 3 To accurately determine circulating concentrations of 25-OH-D without other vitamin D metabolites, a silica Sep-Pak cartridge chromatography can be performed. 4 In this paper, we describe the method used in our laboratory for the assay of 25-OH-D in plasma and serum. The method for vitamin D determination was reported previously in this journal. 5 The assay for 1, 25-dihydroxyvitamin D is described in the following paper.