Atrial fibrillation is associated with alterations in HDL function, metabolism, and particle number
Atrial fibrillation is associated with alterations in HDL function, metabolism, and particle number
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DOI：
10.1007/s00395-019-0735-0
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发表时间：
2019-07-01
影响因子：
9.5
通讯作者：
Buettner, Petra
中科院分区：
文献类型：
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作者：
Trieb, Markus;Kornej, Jelena;Buettner, Petra
房颤（AF）发病率和死亡率的增加与支持该疾病的促纤维化、促血栓形成和促炎症过程有关。高密度脂蛋白（HDL）具有抗炎、抗氧化和抗血栓形成的特性。因此，HDL的功能损害可能与AF的发生或进展有关。我们研究了AF患者和健康对照者的HDL质量和数量指标，包括HDL颗粒数、HDL胆固醇、载脂蛋白（apo）A-I水平、血清淀粉样蛋白A（SAA）含量和HDL胆固醇流出能力，以及apoB去除血清的对氧磷酶活性。从导管消融前的AF患者（n = 91）和年龄和性别匹配的对照受试者（n = 54）中采集血清样本。使用[H-3]-胆固醇标记的J774巨噬细胞在经验证的试验中评估HDL-胆固醇流出能力。卵磷脂-胆固醇酰基转移酶（LCAT）和对氧磷酶的活动进行了评估，使用荧光测定法，SAA水平测定ELISA，和总的和亚类HDL-颗粒数进行了评估，通过核磁共振光谱。采用免疫比浊法测定ApoA-I水平。与健康人相比，AF患者的HDL-胆固醇流出量、HDL-颗粒数、apoA-I水平和LCAT活性显著降低（所有p
Increased morbidity and mortality in atrial fibrillation (AF) are related to the pro-fibrotic, pro-thrombotic, and pro-inflammatory processes that underpin the disease. High-density lipoproteins (HDL) have anti-inflammatory, anti-oxidative, and anti-thrombotic properties. Functional impairment of HDL may, therefore, associate with AF initiation or progression. We studied indices of HDL quality and quantity of AF patients and healthy controls, including HDL-particle number, HDL cholesterol, apolipoprotein (apo) A-I levels, serum amyloid A (SAA) content and HDL-cholesterol efflux capacity, and paraoxonase activity of apoB-depleted serum. Serum samples were collected from AF patients (n=91) before catheter ablation and from age- and sex-matched control subjects (n=54). HDL-cholesterol efflux capacity was assessed in a validated assay using [H-3]-cholesterol-labeled J774 macrophages. Lecithin-cholesterol acyltransferase (LCAT) and paraoxonase activities were assessed using fluorometric assays, SAA levels were determined by ELISA, and total and subclass HDL-particle number was assessed by nuclear magnetic resonance spectroscopy. ApoA-I levels were determined by immunoturbidimetry. HDL-cholesterol efflux capacity, HDL-particle number, apoA-I levels, and LCAT activity were markedly reduced in AF patients when compared to healthy individuals (all p