Comparison of Bone Marrow-derived Mononuclear Cells vs. Mesenchymal Stem Cells in Protecting Random-pattern Skin Flap Survival in Rats
Comparison of Bone Marrow-derived Mononuclear Cells vs. Mesenchymal Stem Cells in Protecting Random-pattern Skin Flap Survival in Rats
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DOI：
10.4172/2157-7633.1000355
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发表时间：
2016-08
期刊：
影响因子：
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通讯作者：
Peng Xu;Yang Lu;Zhenxing Wang;J. Lian;Guangdong Zhou;Wei Liu;Yilin Cao;Wei Li;Jie Zhang
中科院分区：
文献类型：
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作者：
Peng Xu;Yang Lu;Zhenxing Wang;J. Lian;Guangdong Zhou;Wei Liu;Yilin Cao;Wei Li;Jie Zhang
培养的骨髓间充质干细胞（BMSCs）和非培养的骨髓单核细胞（BM-MNCs）均能提高随机皮瓣的存活率。然而，事先培养的骨髓细胞是否有益于这种治疗尚不清楚。在本研究中，我们在随机皮瓣大鼠模型中比较了来自相同骨髓抽吸物的骨髓间充质干细胞和骨髓间充质干细胞的保护作用。bm - mnc组和bmscc组的皮瓣平均存活率分别为71.6±8.4%和66.2±3.1%，均显著高于对照组（55.9±3.4%）。通过血流灌注分析和血管密度测定证实其保护作用。然而，在细胞移植组之间没有观察到显著差异。这些结果表明，目前的骨髓间充质干细胞预培养方法在皮瓣保护方面没有治疗效果。因此，在临床环境中，不进行预培养的BM-MNCs可能更可行。
Both cultured Bone Marrow Mesenchymal Stem Cells (BMSCs) and non-cultured Bone Marrow Mononuclear Cells (BM-MNCs) can improve random-pattern skin flap survival. However, whether prior culture expansion of bone marrow cells is beneficial for this therapy remains unclear. In the current study, the protective effects of BM-MNCs and BMSCs derived from identical bone marrow aspirates were compared in a random-pattern skin flap rat model. The mean skin flap survival rates were 71.6 ± 8.4% in the BM-MNC-treated group and 66.2 ± 3.1% in the BMSCtreated group, both of which were significantly higher than the control group (55.9 ± 3.4%). The protective effects were confirmed by blood perfusion analysis and vessel density assay. However, no significant difference was observed between the cell transplanted groups. These results indicate that the current method for pre-culture of BMSCs does not bring therapeutic benefits in skin flap protection. Therefore, BM-MNCs without pre-culture might be more practicable in the clinical setting.