Two homologous LHY pairs negatively control soybean drought tolerance by repressing the abscisic acid responses
Two homologous LHY pairs negatively control soybean drought tolerance by repressing the abscisic acid responses
复制标题
两个同源 LHY 对通过抑制脱落酸反应负向控制大豆耐旱性
DOI：
10.1111/nph.17019
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发表时间：
2021
期刊：
影响因子：
9.4
通讯作者：
Li Meina
中科院分区：
文献类型：
--
作者：
Wang Kai;Bu Tiantian;Cheng Qun;Dong Lidong;Su Tong;Chen Zimei;Kong Fanjiang;Gong Zhizhong;Liu Baohui;Li Meina
生物钟在植物开花、植物激素合成和非生物胁迫反应等多种生物过程中发挥着重要作用。在模式植物中，生物钟基因调节干旱胁迫反应的方式已经很好地建立起来，但在作物物种中，如大豆，一种主要的全球作物中，人们所知的相对较少。本文报道了拟南芥中CCA1/LHY的同源基因GmLHYs对大豆抗旱性的负调控作用。4个gmlhys的表达均受干旱诱导，四重突变体gmlhys的耐旱性显著提高。转录组分析表明，gmlhys调控脱落酸（ABA）信号通路以响应耐旱性。遗传解剖表明,两个同源染色体ofLHY1aandLHY1bredundantly控制干旱响应。拟南芥和大豆中flhy1和lhy1的功能表征进一步支持了gmlhys在干旱胁迫下通过ABA信号通路维持细胞稳态的观点。本研究提高了我们对大豆抗旱性分子机制的认识。此外，flhy1a&lhy1b的两个同源物为基因组编辑提供了替代靶标，可以在大豆优良品种中快速产生突变等位基因，以增强其耐旱性。
The circadian clock plays essential roles in diverse plant biological processes, such as flowering, phytohormone biosynthesis and abiotic stress responses. The manner in which circadian clock genes regulate drought stress responses in model plants has been well established, but comparatively little is known in crop species, such as soybean, a major global crop. This paper reports that the core clock components GmLHYs, the orthologues of CCA1/LHY in Arabidopsis, negatively control drought tolerance in soybean.The expressions of fourGmLHYs were all induced by drought, and the quadruple mutants ofGmLHYs demonstrated significantly improved drought tolerance. Transcriptome profiling suggested that the abscisic acid (ABA) signaling pathway is regulated byGmLHYs to respond to drought tolerance.Genetic dissections showed that two homologous pairs ofLHY1aandLHY1bredundantly control the drought response. Functional characterization ofLHY1aandLHY1bin Arabidopsis and soybean further supported the notion thatGmLHYs can maintain cellular homeostasis through the ABA signaling pathway under drought stress.This study improves our understanding of the underlying molecular mechanisms on soybean drought tolerance. Furthermore, the two homologues ofLHY1aandLHY1bprovide alternative targets for genome editing to rapidly generate mutant alleles in elite soybean cultivars to enhance their drought tolerance.