Direct electrochemistry and electrocatalysis of hemoglobin on a glassy carbon electrode modified with poly(ethylene glycol diglycidyl ether) and gold nanoparticles on a quaternized cellulose support. A sensor for hydrogen peroxide and nitric oxide
Direct electrochemistry and electrocatalysis of hemoglobin on a glassy carbon electrode modified with poly(ethylene glycol diglycidyl ether) and gold nanoparticles on a quaternized cellulose support. A sensor for hydrogen peroxide and nitric oxide
复制标题
在季铵化纤维素载体上用聚乙二醇二缩水甘油醚和金纳米粒子修饰的玻碳电极上对血红蛋白进行直接电化学和电催化。
DOI：
10.1007/s00604-014-1228-3
复制
发表时间：
2014-10-01
影响因子：
5.7
通讯作者：
Feng, Bo
中科院分区：
文献类型：
--
作者：
Li, Fangping;Nie, Mingzhe;Feng, Bo
摘要以季铵化纤维素为载体，聚乙二醇二缩水甘油醚（PEGDGE）为膜，制备了金纳米粒子（Au-NPs）修饰玻碳电极，并将血红蛋白（Hb）固定在Au-NPs上。采用紫外-可见光谱、扫描电子显微镜和电化学阻抗谱对传感膜进行了表征。在Au@Qc/PEGDGE膜中的Hb的循环伏安法揭示了蛋白质血红素Fe（III）/Fe（II）氧化还原对在约−0.333 V（vs. SCE）处的一对明确的准可逆峰。该传感膜对一氧化氮和过氧化氢的还原也表现出良好的电催化活性。在0.9 ~ 160 μM范围内，该传感器的电流响应与一氧化氮浓度呈线性关系，检测限低至12 nM（3σ）。对过氧化氢的响应在59 nM至4.6 μM浓度范围内呈线性，检测限为16 nM（3σ）。该生物传感器具有灵敏度高、重现性好、长期稳定等特点。
图基于Au@Qc NPs /聚乙二醇二缩水甘油醚复合膜固定血红蛋白的电化学生物传感器。
AbstractA glassy carbon electrode was modified with gold nanoparticles (Au-NPs) on a quaternized cellulose support in a film composed of poly(ethylene glycol diglycidyl ether) (PEGDGE), and Hb was immobilized on the Au-NPs. The sensor film was characterized by UV–vis spectra, scanning electron microscopy, and electrochemical impedance spectroscopy. Cyclic voltammetry of the Hb in the Au@Qc/PEGDGE film revealed a pair of well-defined and quasi reversible peaks for the protein heme Fe(III)/Fe(II) redox couple at about −0.333 V (vs. SCE). The sensor film also exhibited good electrocatalytic activity for the reduction of nitric oxide and hydrogen peroxide. The amperometric response of the biosensor depends linearly on the concentration of nitric oxide in the 0.9 to 160 μM range, and the detection limit is as low as 12 nM (at 3σ). The response to hydrogen peroxide is linear in the 59 nM to 4.6 μM concentration range, and the detection limit is 16 nM (at 3σ). This biosensor is sensitive, reproducible, and long-term stable.
FigureAn electrochemical biosensor based on the immobilization of hemoglobin in Au@Qc NPs /Poly ethylene glycol diglycidyl ether composite film is developed.