Influences of small-scale oscillations on growth inhibition and ultrastructural changes of Microcystis cells
Influences of small-scale oscillations on growth inhibition and ultrastructural changes of Microcystis cells
复制标题
小尺度振荡对微囊藻细胞生长抑制和超微结构变化的影响
DOI：
10.1080/10934529.2018.1530329
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Park Ho-Dong
中科院分区：
文献类型：
--
作者：
Han Jisun;Jeon Bong-Seok;Mizuno Katsunori;Yoshida Kenji;Park Ho-Dong
我们研究了小尺度振荡(SSO)对有毒微囊藻细胞的影响。振荡器由两轴硅片制成，直径为∼40 mm，频率为2.5 Hz，转速为15 0 。SSO能有效地抑制微囊藻的生长。振荡组未观察到微囊藻毒素释放，而细胞密度几乎没有增加。振荡组细胞大小和形态与对照组无明显差异。然而，振荡组的Chl.a和微囊藻毒素的细胞配额是对照组的一半。最重要的是，在振荡组中观察到了许多大尺寸的孔洞，很少观察到层状的长线状类囊体。因此，SSO对微囊藻的生长有很强的抑制作用，且对细胞膜无损伤，对微囊藻毒素的释放无明显影响。
We investigated the effects of small-scale oscillation (SSO) on toxicMicrocystiscells. The oscillating device was made of silicon with two axes that had a diameter of ∼40 mm, and a frequency of 2.5 Hz was observed at 150 rpm. The SSO was effective in inhibitingMicrocystisgrowth. Microcystin release was not observed, whereas cell density barely increased in the oscillating group. Cell size and morphology of the oscillating group were no different from the control group. However, cell quotas of chl.a and microcystin in the oscillating group were half the level of the control group. Crucially, a number of large-sized holes were observed and layered long linear thylakoids were rarely observed in the oscillating group. Therefore, SSO was found to be very effective inMicrocystisgrowth inhibition, and it caused ultrastructural changes without damage to the cell membrane and subsequent microcystin release.