The effects of 1α,25-dihydroxyvitamin D3 on the expression of DNA replication genes
The effects of 1α,25-dihydroxyvitamin D3 on the expression of DNA replication genes
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DOI：
10.1359/jbmr.0301204
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发表时间：
2004-01-01
影响因子：
6.2
通讯作者：
Verstuyf, A
中科院分区：
文献类型：
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作者：
Eelen, G;Verlinden, L;Verstuyf, A
为了鉴定1，25（OH）（2）D-3抗增殖作用中的关键基因，对MC 3 T3-E1小鼠成骨细胞进行cDNA微阵列分析。11个E2 F驱动的DNA复制基因被1，25（OH）（2）D-3下调。这些结果在不同细胞类型中通过定量RT-PCR得到证实，显示了1，25（OH）（2）D-3这种作用的一般性质。
To identify key genes in the antiproliferative action of 1,25(OH)(2)D-3, MC3T3-E1 mouse osteoblasts were subjected to cDNA microarray analyses. Eleven E2F-driven DNA replication genes were downregulated by 1,25(OH)(2)D-3. These results were confirmed by quantitative RT-PCR in different cell types, showing the general nature of this action of 1,25(OH)(2)D-3.