Genome-wide association mapping reveals novel sources of resistance to northern corn leaf blight in maize.
Genome-wide association mapping reveals novel sources of resistance to northern corn leaf blight in maize.
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DOI：
10.1186/s12870-015-0589-z
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发表时间：
2015-08-20
影响因子：
5.3
通讯作者：
Yan J
中科院分区：
文献类型：
--
作者：
Ding J;Ali F;Chen G;Li H;Mahuku G;Yang N;Narro L;Magorokosho C;Makumbi D;Yan J
玉米白叶枯病是由玉米大斑病菌引起的一种破坏性病害。利用寄主抗性来减少NCLB对玉米生产的不利影响是最具成本效益和吸引力的病害管理策略。然而，这需要鉴定和利用在不同环境中有效的稳定抗性基因。我们评估了由999个自交系组成的不同玉米群体在不同环境中对NCLB的抗性。利用56,110个单核苷酸多态标记对玉米进行了全基因组关联分析，以确定玉米抗NCLB相关基因组区域。单标记和基于单倍型的关联，以及安德森-达林检验，确定了与NCLB抗性显著相关的等位基因。单标记和基于单倍型的关联作图分别确定了12个和10个与NCLB抗性显著相关的基因座。此外，通过将群体划分为3个亚群并进行Anderson-Darling检验，共检测到81个基因，其中12个基因与植物防御有关。利用这三种分析鉴定出了相同的防御基因。利用由999个不同品系组成的组合小组，在多种环境下对NCLB进行抗性鉴定，鉴定出大量的抗性品系，可作为玉米育种的可靠抗源。全基因组关联研究表明，NCLB抗性是一个复杂的性状，受许多微效基因控制，但效应相对较低。在相同的背景下聚合这些基因可能会导致对NCLB的稳定抗性。本文的在线版本(doi：10.1186/s12870-0150589-z)包含补充材料，授权用户可以使用。
Northern corn leaf blight (NCLB) caused by Exserohilum turcicum is a destructive disease in maize. Using host resistance to minimize the detrimental effects of NCLB on maize productivity is the most cost-effective and appealing disease management strategy. However, this requires the identification and use of stable resistance genes that are effective across different environments. We evaluated a diverse maize population comprised of 999 inbred lines across different environments for resistance to NCLB. To identify genomic regions associated with NCLB resistance in maize, a genome-wide association analysis was conducted using 56,110 single-nucleotide polymorphism markers. Single-marker and haplotype-based associations, as well as Anderson-Darling tests, identified alleles significantly associated with NCLB resistance. The single-marker and haplotype-based association mappings identified twelve and ten loci (genes), respectively, that were significantly associated with resistance to NCLB. Additionally, by dividing the population into three subgroups and performing Anderson-Darling tests, eighty one genes were detected, and twelve of them were related to plant defense. Identical defense genes were identified using the three analyses. An association panel including 999 diverse lines was evaluated for resistance to NCLB in multiple environments, and a large number of resistant lines were identified and can be used as reliable resistance resource in maize breeding program. Genome-wide association study reveals that NCLB resistance is a complex trait which is under the control of many minor genes with relatively low effects. Pyramiding these genes in the same background is likely to result in stable resistance to NCLB. The online version of this article (doi:10.1186/s12870-015-0589-z) contains supplementary material, which is available to authorized users.