Method for quantification of morphine and its 3-and 6-glucuronides, codeine, codeine glucuronide and 6-monoacetylmorphine in human blood by liquid chromatography-electrospray mass spectrometry for routine analysis in forensic toxicology
Method for quantification of morphine and its 3-and 6-glucuronides, codeine, codeine glucuronide and 6-monoacetylmorphine in human blood by liquid chromatography-electrospray mass spectrometry for routine analysis in forensic toxicology
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DOI：
10.1016/s0021-9673(99)00750-5
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发表时间：
1999-08-27
影响因子：
4.1
通讯作者：
Mangin, P
中科院分区：
文献类型：
--
作者：
Dienes-Nagy, A;Rivier, L;Mangin, P
同时测定体液中阿片类药物及其葡萄糖醛酸苷在海洛因中毒的法医学鉴定中具有重要的实际意义。本文报道了一种基于液相色谱-电喷雾电离质谱的吗啡及其3-和6-葡萄糖醛酸苷、可待因、可待因葡萄糖醛酸苷和6-单乙酰吗啡（6-MAM）的选择性和灵敏度的定量方法。在C-8柱上进行固相萃取后，在人尸检全血中分析药物。在ODS柱上在乙腈中进行分离（分析时间15分钟）。对于定量分析，使用每种化合物的氘代类似物作为内标。在选择分子离子进行定量的情况下，应用选择离子监测。吗啡和B-MAM的定量限为0.5 ng/ml，吗啡的6-葡萄糖醛酸苷、可待因-6-葡萄糖醛酸苷和可待因的定量限为1 ng/ml，吗啡的3-葡萄糖醛酸苷的定量限为5 ng/ml。(C)1999 Elsevier Science B. V.保留所有权利。
Simultaneous determination of opiates and their glucuronides in body fluids has a great practical interest in the forensic assessment of heroin intoxication. A selective and sensitive method for quantification of morphine and its 3- and 6-glucuronides, codeine, codeine glucuronide and 6-monoacetylmorphine (6-MAM) based on liquid chromatography-electrospray ionisation mass spectrometry is described. The drugs were analysed in human autopsy whole blood after solid-phase extraction on a C-8 cartridge. The separation was performed on an ODS column in acetonitrile (analysis time 15 min). For the quantitative analysis, deuterated analogues of each compound were used as internal standards. Selected-ion monitoring was applied where the molecular ion was chosen for quantification. The limits of quantification were 0.5 ng/ml for morphine and B-MAM and 1 ng/ml for the 6-glucuronide of morphine, codeine-6-glucuronide and codeine and 5 ng/ml for the 3-glucuronide of morphine. (C) 1999 Elsevier Science B.V. All rights reserved.